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A library of 27 murine monoclonal antibodies was obtained by using human liver and heart ferritins as immunogens. The specificity of the antibodies for the two ferritins and their subunits was studied with five different methods. The antibodies elicited by the liver ferritin bound preferentially the immunogen and were specific for the L subunit. Some antibodies elicited by the heart ferritin had characteristics similar to the anti-liver antibodies, other ones bound preferentially the heart over the liver ferritin and were specific for the H subunit. Only two antibodies were able to bind both ferritins and subunits. Some anti-H and anti-L chain antibodies were used to develop and compare four types of immunoassay to quantitate isoferritins. The results indicate that heart ferritin is immunologically more heterogeneous than liver, the H and L subunits having large immunological differences with few, if any, identical epitopes; and that that the architecture of the immunoassays have a strong influence on the crossreactivity of the antibodies with the two isoferritins, probably because H and L chains are not arranged randomly in the assembled protein.
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P Arosio, and
C Iacobello, and
G Ruggeri, and
L Capucci, and
E Brocchi, and
F De Simone, and
D Gamba, and
E Gabri, and
S Levi
August 1988,
Molecular endocrinology (Baltimore, Md.),
A Luzzago, and
P Arosio, and
C Iacobello, and
G Ruggeri, and
L Capucci, and
E Brocchi, and
F De Simone, and
D Gamba, and
E Gabri, and
S Levi
April 1985,
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A Luzzago, and
P Arosio, and
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G Ruggeri, and
L Capucci, and
E Brocchi, and
F De Simone, and
D Gamba, and
E Gabri, and
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January 1982,
Annals of the New York Academy of Sciences,
A Luzzago, and
P Arosio, and
C Iacobello, and
G Ruggeri, and
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F De Simone, and
D Gamba, and
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S Levi
January 1997,
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A Luzzago, and
P Arosio, and
C Iacobello, and
G Ruggeri, and
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June 1993,
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A Luzzago, and
P Arosio, and
C Iacobello, and
G Ruggeri, and
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F De Simone, and
D Gamba, and
E Gabri, and
S Levi
February 1984,
FEBS letters,
A Luzzago, and
P Arosio, and
C Iacobello, and
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February 1996,
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E Gabri, and
S Levi
October 1987,
Investigative ophthalmology & visual science,
A Luzzago, and
P Arosio, and
C Iacobello, and
G Ruggeri, and
L Capucci, and
E Brocchi, and
F De Simone, and
D Gamba, and
E Gabri, and
S Levi
January 1987,
Gastroenterology,
A Luzzago, and
P Arosio, and
C Iacobello, and
G Ruggeri, and
L Capucci, and
E Brocchi, and
F De Simone, and
D Gamba, and
E Gabri, and
S Levi
June 1994,
Toxicon : official journal of the International Society on Toxinology,
