[CD226 knockout aggravates radiation-induced liver injury in mice]. 2019

Wei Hu, and Dongliang Zhang, and Gengyao Zhou, and Jinxue Zhang, and Xin Yi, and Jiangang Xie, and Yuan Zhang, and Kun Yang, and Ran Zhuang
Department of Immunology, Transplant Immunology Laboratory, School of Basic Medical Sciences, Air Force Military Medical University, Xi'an 710032, China.

Objective To investigate the effect of CD226 knockout (KO) on radiation-induced liver injury in mice. Methods Wild-type (WT) and CD226KO mice were randomly divided into naive group and irradiated group. The mice in the irradiated group were exposed to an 8 Gy of 60Co γ-radiation. The survival rate was observed and body mass loss was measured. The pathological changes in the liver and colon were examined by HE staining, and the mRNA levels of inflammatory factors, including inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), IL-6, IL-12p40, tumor necrosis factor α (TNF-α) and monocyte chemoattractant protein-1 (MCP-1) were detected by real-time quantitative PCR. Results Compared with the irradiated WT mice, the irradiated CD226KO mice had increased body mass loss and decreased survival rate. In the irradiated CD226KO mice, the liver injury was more serious, the liver index (liver mass/body mass) was reduced, and the expressive levels of iNOS, IL-1β, IL-6, IL-12p40, TNF-α and MCP-1 were enhanced; whereas, the colitis was less serious, and the expressive levels of inflammatory factors were down-regulated. Conclusion The deficiency of CD226 can decrease the survival rate of irradiated mice, which is associated with the aggravated liver injury.

UI MeSH Term Description Entries
D008099 Liver A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances. Livers
D008810 Mice, Inbred C57BL One of the first INBRED MOUSE STRAINS to be sequenced. This strain is commonly used as genetic background for transgenic mouse models. Refractory to many tumors, this strain is also preferred model for studying role of genetic variations in development of diseases. Mice, C57BL,Mouse, C57BL,Mouse, Inbred C57BL,C57BL Mice,C57BL Mice, Inbred,C57BL Mouse,C57BL Mouse, Inbred,Inbred C57BL Mice,Inbred C57BL Mouse
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000945 Antigens, Differentiation, T-Lymphocyte Antigens expressed on the cell membrane of T-lymphocytes during differentiation, activation, and normal and neoplastic transformation. Their phenotypic characterization is important in differential diagnosis and studies of thymic ontogeny and T-cell function. Antigens, Differentiation, T-Cell,Differentiation Antigens, T-Cell,L3T4 Antigens,Leu Antigens, T-Lymphocyte,T-Cell Differentiation Antigens,T-Lymphocyte Differentiation Antigens,T6 Antigens,Antigens, Differentiation, T Lymphocyte,Differentiation Antigens, T Lymphocyte,Antigens, L3T4,Antigens, T-Cell Differentiation,Antigens, T-Lymphocyte Differentiation,Antigens, T-Lymphocyte Leu,Antigens, T6,Differentiation Antigens, T Cell,Differentiation Antigens, T-Lymphocyte,Leu Antigens, T Lymphocyte,T Cell Differentiation Antigens,T Lymphocyte Differentiation Antigens,T-Lymphocyte Leu Antigens
D014409 Tumor Necrosis Factor-alpha Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS. Cachectin,TNF-alpha,Tumor Necrosis Factor Ligand Superfamily Member 2,Cachectin-Tumor Necrosis Factor,TNF Superfamily, Member 2,TNFalpha,Tumor Necrosis Factor,Cachectin Tumor Necrosis Factor,Tumor Necrosis Factor alpha
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus
D053583 Interleukin-1beta An interleukin-1 subtype that is synthesized as an inactive membrane-bound pro-protein. Proteolytic processing of the precursor form by CASPASE 1 results in release of the active form of interleukin-1beta from the membrane. IL-1 beta,Catabolin,Interleukin-1 beta,Interleukin 1 beta,Interleukin 1beta

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