Biomaterial Database

A mechanized device for mounting histological tissue sections. 2019

Osama Habbal, and Ahmad Farhat, and Reem Khalil

American University of Sharjah, Department of Mechanical Engineering, Sharjah, UAE.

Traditional methods for mounting tissue sections onto slides are suboptimal as the amount of labor required quickly multiplies with increasing number of samples. Methods to accelerate the tissue mounting process while reducing the associated risk of tissue damage are needed. We designed and 3D printed a mechanized device with an inclined platform used to mount tissue sections onto slides in buffer solution. The main advantage of this design is to reduce the time required for mounting sections as well as minimize the possibility of damaging delicate or thin tissue sections. Using our device, we illustrate and describe in detail the steps required to mount smaller coronally cut mouse brain sections, as well as bigger tangentially cut ferret brain sections. This method's efficiency was assessed by comparing the time required to mount an entire slide of ferret brain sections using our method and the conventional method. Using our device reduced the tissue mounting time by 60%. Compared to existing conventional tissue mounting methods, our device is a simple and user friendly alternative that substantially reduces the time required to mount tissue sections while preserving tissue section quality. Using our device can streamline histological processing and prove to be especially useful for a variety of tissue types as the platform was designed to accommodate different size microscope slides, and thus use for varying tissue section sizes.

UI	MeSH Term	Description	Entries
D008810	Mice, Inbred C57BL	One of the first INBRED MOUSE STRAINS to be sequenced. This strain is commonly used as genetic background for transgenic mouse models. Refractory to many tumors, this strain is also preferred model for studying role of genetic variations in development of diseases.	Mice, C57BL,Mouse, C57BL,Mouse, Inbred C57BL,C57BL Mice,C57BL Mice, Inbred,C57BL Mouse,C57BL Mouse, Inbred,Inbred C57BL Mice,Inbred C57BL Mouse
D009488	Neurosciences	The scientific disciplines concerned with the embryology, anatomy, physiology, biochemistry, pharmacology, etc., of the nervous system.	Neuroscience
D001921	Brain	The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.	Encephalon
D004867	Equipment Design	Methods and patterns of fabricating machines and related hardware.	Design, Equipment,Device Design,Medical Device Design,Design, Medical Device,Designs, Medical Device,Device Design, Medical,Device Designs, Medical,Medical Device Designs,Design, Device,Designs, Device,Designs, Equipment,Device Designs,Equipment Designs
D005289	Ferrets	Semidomesticated variety of European polecat much used for hunting RODENTS and/or RABBITS and as a laboratory animal. It is in the subfamily Mustelinae, family MUSTELIDAE.	Domestic Polecat,Domestic Polecats,European Polecat,European Polecats,Ferret,Mustela putorius,Mustela putorius furo,Polecat, Domestic,Polecat, European,Polecats, Domestic,Polecats, European
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D016591	Histocytological Preparation Techniques	Methods of preparing cells or tissues for examination and study of their origin, structure, function, or pathology. The methods include preservation, fixation, sectioning, staining, replica, or other technique to allow for viewing using a microscope.	Cytohistological Preparation Techniques,Cytohistologic Preparation Technics,Cytohistologic Preparation Technique,Cytohistologic Preparation Techniques,Cytohistological Preparation Technic,Cytohistological Preparation Technics,Cytohistological Preparation Technique,Histocytologic Preparation Technic,Histocytologic Preparation Technics,Histocytologic Preparation Technique,Histocytologic Preparation Techniques,Histocytological Preparation Technic,Histocytological Preparation Technics,Histocytological Preparation Technique,Preparation Technic, Cytohistologic,Preparation Technic, Cytohistological,Preparation Technic, Histocytologic,Preparation Technic, Histocytological,Preparation Technics, Cytohistologic,Preparation Technics, Cytohistological,Preparation Technics, Histocytologic,Preparation Technics, Histocytological,Preparation Technique, Cytohistologic,Preparation Technique, Cytohistological,Preparation Technique, Histocytologic,Preparation Technique, Histocytological,Preparation Techniques, Cytohistologic,Preparation Techniques, Cytohistological,Preparation Techniques, Histocytologic,Preparation Techniques, Histocytological,Technic, Cytohistologic Preparation,Technic, Cytohistological Preparation,Technic, Histocytologic Preparation,Technic, Histocytological Preparation,Technics, Cytohistologic Preparation,Technics, Cytohistological Preparation,Technics, Histocytologic Preparation,Technics, Histocytological Preparation,Technique, Cytohistologic Preparation,Technique, Cytohistological Preparation,Technique, Histocytologic Preparation,Technique, Histocytological Preparation,Techniques, Cytohistologic Preparation,Techniques, Cytohistological Preparation,Techniques, Histocytologic Preparation,Techniques, Histocytological Preparation,Cytohistologic Preparation Technic
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus
D066330	Printing, Three-Dimensional	Process for making, building or constructing a physical object from a three-dimensional digital model by laying down many successive thin layers of building material.	3-D Printing,3-Dimensional Printing,3D Printing,Three-Dimensional Printing,3 D Printing,3 Dimensional Printing,3-D Printings,3-Dimensional Printings,3D Printings,Printing, 3-D,Printing, 3-Dimensional,Printing, 3D,Printing, Three Dimensional,Printings, 3-D,Printings, 3-Dimensional,Printings, 3D,Printings, Three-Dimensional,Three Dimensional Printing,Three-Dimensional Printings