Biomaterial Database

Linker polypeptides of the phycobilisome from the cyanobacterium Mastigocladus laminosus. II. Amino-acid sequences and functions. 1986

P Füglistaller, and F Suter, and H Zuber

The complete primary structure of an 80-residue linker polypeptide, LR(C)8.9, from the phycobilisome of the cyanobacterium Mastigocladus laminosus was determined as well as the 44 N-terminal residues of the two linker polypeptides LR34.5,PEC and LR34.5,PC and the 114 C-terminal residues of LR34.5,PEC. A brief description of the structure determination and an extensive discussion of the relationships of these polypeptides have been published recently (Füglistaller, P., Suter, F. & Zuber, H. (1985) Biol. Chem. Hoppe-Seyler 366, 993-1001). In this paper we report in detail about the elucidation of the primary structures. Limited digestion of the hexameric phycobiliprotein-linker polypeptide complex (alpha PEC beta PEC)6LR34.5,PEC with various proteases resulted in a linker polypeptide diminished by a 1-5 kDa segment, while the phycobiliproteins remained intact. By N-terminal sequence analysis of the residual part of the linker polypeptide in the complex, LR34.5-5,PEC, it was concluded that the C-terminus of the polypeptide had been attacked by the proteases. This C-terminal part of the protein influences the hexamer formation of phycoerythrocyanin (PEC) and is responsible for the linkage between two phycobiliprotein hexamers. From the function of the C-terminal segment of LR34.5,PEC and its homology to the LR(C)8.9 polypeptide, it was concluded that LR(C)8.9 is located at the end of the peripheral phycobilisomal rods distal to the allophycocyanin core.

UI	MeSH Term	Description	Entries
D010940	Plant Proteins	Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which PLANT PROTEINS, DIETARY is available.	Plant Protein,Protein, Plant,Proteins, Plant
D000458	Cyanobacteria	A phylum of oxygenic photosynthetic bacteria comprised of unicellular to multicellular bacteria possessing CHLOROPHYLL a and carrying out oxygenic PHOTOSYNTHESIS. Cyanobacteria are the only known organisms capable of fixing both CARBON DIOXIDE (in the presence of light) and NITROGEN. Cell morphology can include nitrogen-fixing heterocysts and/or resting cells called akinetes. Formerly called blue-green algae, cyanobacteria were traditionally treated as ALGAE.	Algae, Blue-Green,Blue-Green Bacteria,Cyanophyceae,Algae, Blue Green,Bacteria, Blue Green,Bacteria, Blue-Green,Blue Green Algae,Blue Green Bacteria,Blue-Green Algae
D000595	Amino Acid Sequence	The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.	Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D001426	Bacterial Proteins	Proteins found in any species of bacterium.	Bacterial Gene Products,Bacterial Gene Proteins,Gene Products, Bacterial,Bacterial Gene Product,Bacterial Gene Protein,Bacterial Protein,Gene Product, Bacterial,Gene Protein, Bacterial,Gene Proteins, Bacterial,Protein, Bacterial,Proteins, Bacterial
D045342	Light-Harvesting Protein Complexes	Complexes containing CHLOROPHYLL and other photosensitive molecules. They serve to capture energy in the form of PHOTONS and are generally found as components of the PHOTOSYSTEM I PROTEIN COMPLEX or the PHOTOSYSTEM II PROTEIN COMPLEX.	Antenna Complexes, Light-Harvesting,Light-Harvesting Antenna Complexes,Light-Harvesting Chlorophyll Protein,Light-Harvesting Chlorophyll Protein Complexes,Antenna Complexes, Light Harvesting,Chlorophyll Protein, Light-Harvesting,Complexes, Light-Harvesting Antenna,Complexes, Light-Harvesting Protein,Light Harvesting Antenna Complexes,Light Harvesting Chlorophyll Protein,Light Harvesting Chlorophyll Protein Complexes,Light Harvesting Protein Complexes,Protein Complexes, Light-Harvesting
D045524	Phycobilisomes	Light energy harvesting structures attached to the THYLAKOID MEMBRANES of CYANOBACTERIA and RED ALGAE. These multiprotein complexes contain pigments (PHYCOBILIPROTEINS) that transfer light energy to chlorophyll a.	Phycobilisome