Overfeeding a High-Fat Diet Promotes Sex-Specific Alterations on the Gut Microbiota of the Zebrafish (Danio rerio). 2019

Erick Navarro-Barrón, and Crisantema Hernández, and Raúl Llera-Herrera, and Alejandra García-Gasca, and Bruno Gómez-Gil
1 CIAD, A.C. Mazatlán Unit for Aquaculture, Mazatlán, Mexico.

Diet modulates the gut microbiota and is one of the main factors promoting obesity and overweight. In the present study, we investigated the effect of a high-fat diet (HFD) on the gut microbiota of the zebrafish (Danio rerio). Fish were separated into three groups and fed in different regimes: low fat, high fat, and high fat overfed; the experiments were performed on males and females separately. We analyzed more than 2.6 million sequences of variable region V3 of the 16S rRNA gene generated by the Illumina Miniseq platform, clustered to 97% similarity with vsearch and classified with the EzBioCloud database. The weight gain, condition factor (K), and body mass index were calculated as indicators of obesity. Multivariate analysis (PERMANOVA and ANOSIM) and diversity indices (Shannon and Dominance) revealed that overfeeding a HFD disturbs the gut microbiota differently in males and females suggesting that sex is a significant factor (p < 0.05) for the composition of the gut microbiota of zebrafish. The results also indicate that a HFD provided in a basal caloric regime does not promote obesity or alterations in the gut microbiota.

UI MeSH Term Description Entries
D008297 Male Males
D011897 Random Allocation A process involving chance used in therapeutic trials or other research endeavor for allocating experimental subjects, human or animal, between treatment and control groups, or among treatment groups. It may also apply to experiments on inanimate objects. Randomization,Allocation, Random
D004435 Eating The consumption of edible substances. Dietary Intake,Feed Intake,Food Intake,Macronutrient Intake,Micronutrient Intake,Nutrient Intake,Nutritional Intake,Ingestion,Dietary Intakes,Feed Intakes,Intake, Dietary,Intake, Feed,Intake, Food,Intake, Macronutrient,Intake, Micronutrient,Intake, Nutrient,Intake, Nutritional,Macronutrient Intakes,Micronutrient Intakes,Nutrient Intakes,Nutritional Intakes
D005260 Female Females
D000069196 Gastrointestinal Microbiome All of the microbial organisms that naturally exist within the GASTROINTESTINAL TRACT. Enteric Bacteria,Gastric Microbiome,Gastrointestinal Flora,Gastrointestinal Microbial Community,Gastrointestinal Microbiota,Gastrointestinal Microflora,Gut Flora,Gut Microbiome,Gut Microbiota,Gut Microflora,Intestinal Flora,Intestinal Microbiome,Intestinal Microbiota,Intestinal Microflora,Bacteria, Enteric,Flora, Gastrointestinal,Flora, Gut,Flora, Intestinal,Gastric Microbiomes,Gastrointestinal Microbial Communities,Gastrointestinal Microbiomes,Gastrointestinal Microbiotas,Gut Microbiomes,Gut Microbiotas,Intestinal Microbiomes,Intestinal Microbiotas,Microbial Community, Gastrointestinal,Microbiome, Gastric,Microbiome, Gastrointestinal,Microbiome, Gut,Microbiome, Intestinal,Microbiota, Gastrointestinal,Microbiota, Gut,Microbiota, Intestinal,Microflora, Gastrointestinal,Microflora, Gut,Microflora, Intestinal
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012737 Sex Factors Maleness or femaleness as a constituent element or influence contributing to the production of a result. It may be applicable to the cause or effect of a circumstance. It is used with human or animal concepts but should be differentiated from SEX CHARACTERISTICS, anatomical or physiological manifestations of sex, and from SEX DISTRIBUTION, the number of males and females in given circumstances. Factor, Sex,Factors, Sex,Sex Factor
D015027 Zebrafish An exotic species of the family CYPRINIDAE, originally from Asia, that has been introduced in North America. Zebrafish is a model organism for drug assay and cancer research. Brachydanio rerio,Danio rerio,B. rerio,D. rerio,Zebra Fish,Zebra Fishes,Zebra danio,Zebrafishes,D. rerios,Fishes, Zebra,Zebra danios,danio, Zebra
D059305 Diet, High-Fat Consumption of excessive DIETARY FATS. Diet, High Fat,Diets, High Fat,Diets, High-Fat,High Fat Diet,High Fat Diets,High-Fat Diet,High-Fat Diets

Related Publications

Erick Navarro-Barrón, and Crisantema Hernández, and Raúl Llera-Herrera, and Alejandra García-Gasca, and Bruno Gómez-Gil
May 2008, Heredity,
Erick Navarro-Barrón, and Crisantema Hernández, and Raúl Llera-Herrera, and Alejandra García-Gasca, and Bruno Gómez-Gil
February 2002, Genetics,
Erick Navarro-Barrón, and Crisantema Hernández, and Raúl Llera-Herrera, and Alejandra García-Gasca, and Bruno Gómez-Gil
October 2022, Ecology and evolution,
Erick Navarro-Barrón, and Crisantema Hernández, and Raúl Llera-Herrera, and Alejandra García-Gasca, and Bruno Gómez-Gil
October 2020, Environmental pollution (Barking, Essex : 1987),
Erick Navarro-Barrón, and Crisantema Hernández, and Raúl Llera-Herrera, and Alejandra García-Gasca, and Bruno Gómez-Gil
March 2024, International journal of molecular sciences,
Erick Navarro-Barrón, and Crisantema Hernández, and Raúl Llera-Herrera, and Alejandra García-Gasca, and Bruno Gómez-Gil
October 2023, Journal of fish biology,
Erick Navarro-Barrón, and Crisantema Hernández, and Raúl Llera-Herrera, and Alejandra García-Gasca, and Bruno Gómez-Gil
June 2021, Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology,
Erick Navarro-Barrón, and Crisantema Hernández, and Raúl Llera-Herrera, and Alejandra García-Gasca, and Bruno Gómez-Gil
October 2022, Comparative biochemistry and physiology. Toxicology & pharmacology : CBP,
Erick Navarro-Barrón, and Crisantema Hernández, and Raúl Llera-Herrera, and Alejandra García-Gasca, and Bruno Gómez-Gil
May 1999, Contemporary topics in laboratory animal science,
Erick Navarro-Barrón, and Crisantema Hernández, and Raúl Llera-Herrera, and Alejandra García-Gasca, and Bruno Gómez-Gil
May 2022, Comparative biochemistry and physiology. Toxicology & pharmacology : CBP,
Copied contents to your clipboard!