Sixty six consecutive P. aeruginosa isolates from heterogeneous clinical specimens were subjected to aeruginocine (pyocine) typing and assayed for in vitro protease and elastase production by a simple and reproducible qualitative test. The 45.4% of the clinical isolates were found to be both protease and elastase (P + E +) producers; 40.9% were only protease producers (P + E -) and 13.6% were non producers (P - E -). Aeruginocine code 7777 strains were found to be predominant among P + E + and P + E - types, as 48.2% and 51.7% isolates belonged to the types, respectively, suggesting thereby the virulence of this aeruginocine type in P. aeruginosa infections and the possible association of protease and elastase production with aeruginocine production.