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Alpha-difluoromethylornithine (DFMO), an irreversible inhibitor of ornithine decarboxylase, has been demonstrated to be an effective agent against a variety of parasitic protozoa but not against Leishmania spp. In this report, we show that Leishmania donovani promastigotes in continuous culture are sensitive to the growth inhibitory and cytotoxic effects of DFMO. Incubation of the promastigotes with DFMO obliterates intracellular putrescine pools and depletes spermidine concentrations, which correlates with the onset of growth inhibition. The effects of DFMO on the growth and the intracellular polyamine pools can be reversed completely by the addition of 10 microM putrescine to the culture medium. These results suggest that the treatment of leishmaniasis may be amenable to chemotherapeutic manipulation by DFMO.
K Kaur, and
K Emmett, and
P P McCann, and
A Sjoerdsma, and
B Ullman
October 1986,
The Indian journal of medical research,
K Kaur, and
K Emmett, and
P P McCann, and
A Sjoerdsma, and
B Ullman
July 2008,
Annals of tropical medicine and parasitology,
K Kaur, and
K Emmett, and
P P McCann, and
A Sjoerdsma, and
B Ullman
August 1995,
Experimental parasitology,
K Kaur, and
K Emmett, and
P P McCann, and
A Sjoerdsma, and
B Ullman
February 1987,
The Indian journal of medical research,
K Kaur, and
K Emmett, and
P P McCann, and
A Sjoerdsma, and
B Ullman
February 1985,
The Journal of protozoology,
K Kaur, and
K Emmett, and
P P McCann, and
A Sjoerdsma, and
B Ullman
May 1994,
Experimental parasitology,
K Kaur, and
K Emmett, and
P P McCann, and
A Sjoerdsma, and
B Ullman
May 1983,
The Journal of experimental medicine,
K Kaur, and
K Emmett, and
P P McCann, and
A Sjoerdsma, and
B Ullman
January 1992,
The Journal of protozoology,
K Kaur, and
K Emmett, and
P P McCann, and
A Sjoerdsma, and
B Ullman
November 1972,
The Journal of protozoology,
K Kaur, and
K Emmett, and
P P McCann, and
A Sjoerdsma, and
B Ullman
July 1984,
Molecular and biochemical parasitology,
