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Four cytochromes P-450 induced by phenobarbital (PB-1--PB-4) and two cytochromes P-450 induced by S-methylcholanthrene (MC-1, MC-2) were purified to electrophoretic homogeneity from rat liver microsomes. The purification procedure involved sequential chromatography on n-aminooctyl-Sepharose 4B, DEAE-Sephacel and hydroxylapatite columns. The spectral and immunochemical properties of the cytochromes P-450 were estimated. All, but MC-1, cytochromes P-450 were found to exist in a low spin state. Using the Ouchterlony double diffusion method, it was shown that all cytochromes P-450 under study can be divided into two groups, i. e., PB-1--PB-2 and PB-3--PB-4, sharing common antigenic determinants inside the groups. High performance liquid chromatography of PB-3 and MC-2 on anion-exchangers yielded two additional peaks from the PB-induced major cytochrome P-450 PB-3 and three peaks from the MC-induced major cytochrome P-450 MC-2. The multiplicity of cytochrome P-450 forms is discussed.
V N Nechaev, and
L R Ptitsyn, and
A A Kabishev, and
L A Riazanova, and
L I Patrushev
March 1976,
The Journal of biological chemistry,
V N Nechaev, and
L R Ptitsyn, and
A A Kabishev, and
L A Riazanova, and
L I Patrushev
January 1975,
Advances in experimental medicine and biology,
V N Nechaev, and
L R Ptitsyn, and
A A Kabishev, and
L A Riazanova, and
L I Patrushev
May 1975,
The Journal of biological chemistry,
V N Nechaev, and
L R Ptitsyn, and
A A Kabishev, and
L A Riazanova, and
L I Patrushev
July 1973,
The Journal of biological chemistry,
V N Nechaev, and
L R Ptitsyn, and
A A Kabishev, and
L A Riazanova, and
L I Patrushev
August 1976,
The Journal of biological chemistry,
V N Nechaev, and
L R Ptitsyn, and
A A Kabishev, and
L A Riazanova, and
L I Patrushev
December 1990,
Brain research,
V N Nechaev, and
L R Ptitsyn, and
A A Kabishev, and
L A Riazanova, and
L I Patrushev
April 1986,
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V N Nechaev, and
L R Ptitsyn, and
A A Kabishev, and
L A Riazanova, and
L I Patrushev
January 1994,
Postepy biochemii,
V N Nechaev, and
L R Ptitsyn, and
A A Kabishev, and
L A Riazanova, and
L I Patrushev
July 1990,
Biokhimiia (Moscow, Russia),
V N Nechaev, and
L R Ptitsyn, and
A A Kabishev, and
L A Riazanova, and
L I Patrushev
April 1976,
European journal of biochemistry,
