Temperature is one of the main factors that influences cardiovascular functioning in ectotherms. Hence this study sought to investigate heart rate responses of a freshwater crab species, Poppiana dentata, to different temperature exposures since the species generally reside in habitats of fluctuating physicochemistry. Heart rates were non-invasively determined in juvenile crabs for three temperature regimes, each over an 8-day session; A: temperature exposures of 26 °C (2 days) to 30 °C (3 days) to 26 °C (3 days), B: 26 °C (2 days) to 32 °C (3 days) to 26 °C (3 days) and C: a control at constant 26 °C. Heart rate variations were significant among the regimes (P < 0.05), with the median heart rate being highest for regime B (74 beats per minute or bpm) during the temperature insult (32 °C), relative to regime A (70 bpm) and the control (64 bpm). Notably, a suppression and inversion of the diurnal cardiac patterns occurred for regimes' A and B crabs respectively, with rates from the highest temperature insult not shifting back to pre-insult levels during recovery (26 °C). It is plausible that P. dentata may have compensatory cardiovascular mechanisms that account for these differential heart rate responses, possibly conveying adaptive strategies in its dynamic habitat conditions.