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The mechanism by which nitrite inhibits outgrowing spores of Bacillus cereus T was examined by using techniques developed earlier for nitrite analogs. The morphological stage of inhibition, cooperativity effects, effect of pH on inhibition, kinetics of protection against iodoacetate incorporation into membrane sulfhydryl groups, and protection against the bacteriocidal effect of carboxymethylation by iodoacetate indicate that nitrite acts as a membrane-directed sulfhydryl agent. The mechanism by which nitrite modifies the chemical reactivity of the sulfhydryl group could be either direct covalent modification or inactivation through communication with another modified membrane component. Profiles of pH effects suggest that the active agent is the protonated form of nitrite. The nitrite concentrations which modify membrane sulfhydryl activity coincide with those which have a bacteriostatic effect. These results are consistent with membrane sulfhydryl modification as a component of the mechanism of nitrite-induced bacteriostasis in this aerobic sporeformer.
G W Buchman, and
J N Hansen
November 1984,
The Journal of biological chemistry,
G W Buchman, and
J N Hansen
June 1974,
Thrombosis et diathesis haemorrhagica,
G W Buchman, and
J N Hansen
May 1955,
Applied microbiology,
G W Buchman, and
J N Hansen
June 1976,
Plant physiology,
G W Buchman, and
J N Hansen
February 1987,
FEBS letters,
G W Buchman, and
J N Hansen
July 1980,
Journal of biochemistry,
G W Buchman, and
J N Hansen
January 1977,
Ukrains'kyi biokhimichnyi zhurnal,
G W Buchman, and
J N Hansen
January 1973,
Biochemistry,
G W Buchman, and
J N Hansen
February 1992,
Biochemistry,
G W Buchman, and
J N Hansen
June 1984,
Clinical biochemistry,
