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Acute exposure to chlorpyrifos and glyphosate induces changes in hemolymph biochemical parameters in the crayfish, Astacus leptodactylus (Eschscholtz, 1823). 2019

Mahdi Banaee, and Mostafa Akhlaghi, and Siyavash Soltanian, and Amin Gholamhosseini, and Hassan Heidarieh, and Mohammad Saeed Fereidouni
Aquaculture Department, Faculty of Natural Resources and the Environment, Behbahan Khatam Alanbia University of Technology, Behbahan, Iran. Electronic address: mahdibanaee2@gmail.com.

Acute and subacute toxicity tests for chlorpyrifos and glyphosate were performed on the crayfish, Astacus leptodactylus. The crayfish were divided into a control group and four experimental groups with exposure from 50.18 to 301.11 μg L-1 of chlorpyrifos for 96 h under semi-static test conditions. In the same experiment, the crayfish were exposed to different concentrations of glyphosate (from 0.0, 8.14 to 13.05 mg L-1) for 96 h. Mortality was recorded, and the median lethal concentrations (LC50) were calculated using probit analysis. The 96 h LC50 values of chlorpyrifos and glyphosate to A. leptodactylus were 49.55 ± 4.66 μg L-1 and 7.83 ± 0.50 mg L-1, respectively. The results showed that chlorpyrifos was a few hundred times more toxic than glyphosate for the crayfish. Then, the crayfish were exposed to different subacute concentrations of chlorpyrifos (12.5 and 25 μg L-1) and glyphosate (2 mg L-1 and 4 mg L-1) for seven days, and their toxicity was evaluated through several hemolymph biochemical parameters. Chlorpyrifos and glyphosate exposure caused a significant (P < 0.01) increase in the activity of lactate dehydrogenase (LDH), and gamma-glutamyl transferase (GGT). The results showed a significant (P < 0.01) decrease in acetylcholinesterase (AChE), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and phenoloxidase (PO) activities in the hemolymph of the crayfish exposed to chlorpyrifos and glyphosate. The total protein and cholesterol levels showed a significant decrease (P < 0.01) in the hemolymph of the crayfish after seven days of exposure to chlorpyrifos and glyphosate. There was a significant increase in triglycerides and glucose levels in treated crayfish. These results show that chlorpyrifos is highly toxic, while glyphosate is moderately toxic to A. leptodactylus. According to the results, subacute concentrations of chlorpyrifos and glyphosate could lead to a collapse of cellular homeostasis and changes in the hemolymph biochemical parameters in the crayfish. Physiological changes caused by exposure to chlorpyrifos and glyphosate in A. leptodactylus may have direct effects on the survival of these organisms.

UI MeSH Term Description Entries
D007306 Insecticides Pesticides designed to control insects that are harmful to man. The insects may be directly harmful, as those acting as disease vectors, or indirectly harmful, as destroyers of crops, food products, or textile fabrics. Insecticide
D003400 Astacoidea A superfamily of various freshwater CRUSTACEA, in the infraorder Astacidea, comprising the crayfish. Common genera include Astacus and Procambarus. Crayfish resemble lobsters, but are usually much smaller. Astacus,Crayfish,Procambarus,Astacoideas,Crayfishs
D004390 Chlorpyrifos An organothiophosphate cholinesterase inhibitor that is used as an insecticide and as an acaricide. Dursban,Lorsban
D005998 Glycine A non-essential amino acid. It is found primarily in gelatin and silk fibroin and used therapeutically as a nutrient. It is also a fast inhibitory neurotransmitter. Aminoacetic Acid,Glycine, Monopotassium Salt,Glycine Carbonate (1:1), Monosodium Salt,Glycine Carbonate (2:1), Monolithium Salt,Glycine Carbonate (2:1), Monopotassium Salt,Glycine Carbonate (2:1), Monosodium Salt,Glycine Hydrochloride,Glycine Hydrochloride (2:1),Glycine Phosphate,Glycine Phosphate (1:1),Glycine Sulfate (3:1),Glycine, Calcium Salt,Glycine, Calcium Salt (2:1),Glycine, Cobalt Salt,Glycine, Copper Salt,Glycine, Monoammonium Salt,Glycine, Monosodium Salt,Glycine, Sodium Hydrogen Carbonate,Acid, Aminoacetic,Calcium Salt Glycine,Cobalt Salt Glycine,Copper Salt Glycine,Hydrochloride, Glycine,Monoammonium Salt Glycine,Monopotassium Salt Glycine,Monosodium Salt Glycine,Phosphate, Glycine,Salt Glycine, Monoammonium,Salt Glycine, Monopotassium,Salt Glycine, Monosodium
D006458 Hemolymph The blood/lymphlike nutrient fluid of some invertebrates. Hemolymphs
D006540 Herbicides Pesticides used to destroy unwanted vegetation, especially various types of weeds, grasses (POACEAE), and woody plants. Some plants develop HERBICIDE RESISTANCE. Algaecide,Algicide,Herbicide,Algaecides,Algicides
D000097797 Glyphosate Active compound in herbicidal formulations that inhibits 3-PHOSPHOSHIKIMATE 1-CARBOXYVINYLTRANSFERASE. Gliphosate,Glyphosate Hydrochloride (2:1),Glyphosate, Calcium Salt,Glyphosate, Calcium Salt (1:1),Glyphosate, Copper (2+) Salt,Glyphosate, Dilithium Salt,Glyphosate, Disodium Salt,Glyphosate, Magnesium Salt,Glyphosate, Magnesium Salt (2:1),Glyphosate, Monoammonium Salt,Glyphosate, Monopotassium Salt,Glyphosate, Monosodium Salt,Glyphosate, Sodium Salt,Glyphosate, Zinc Salt,Yerbimat,Kalach 360 SL,N-(phosphonomethyl)glycine,Roundup
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D014874 Water Pollutants, Chemical Chemical compounds which pollute the water of rivers, streams, lakes, the sea, reservoirs, or other bodies of water. Chemical Water Pollutants,Landfill Leachate,Leachate, Landfill,Pollutants, Chemical Water

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