Detection of buffalo milk adulteration with cow milk by capillary electrophoresis analysis. 2019

Francesca Trimboli, and Nicola Costanzo, and Vincenzo Lopreiato, and Carlotta Ceniti, and Valeria M Morittu, and Antonella Spina, and Domenico Britti
Department of Health Science, Magna Græcia University, Viale Europa, loc. Germaneto, 88100, Catanzaro, Italy. Electronic address: trimboli@unicz.it.

The addition of cow milk during the production of buffalo mozzarella is a common fraud in dairy industries because of the lower price and greater availability of cow milk throughout the year. The aim of this study was to develop a new, rapid, and robust capillary electrophoresis method for detecting and quantifying cow milk in buffalo milk by exploiting cow α-lactalbumin as a marker of adulteration. In particular, a linear calibration curve was generated, using a training set of calibrators consisting of 7 series of 17 buffalo/bovine whey mixtures, obtained after casein precipitation, with increasing percentages of cow whey. The capillary electrophoresis method showed high linearity (R2 = 0.968), repeatability [relative standard deviation (RSD) = 2.11, 3.02, 4.38, and 1.18%, respectively for 5, 10, 20, and 50% of buffalo/bovine whey mixtures], and intermediate precision (RSD = 2.18, 2.49, 5.09, and 3.19%, respectively, for 5, 10, 20, and 50% buffalo/bovine whey mixtures). Moreover, the minimum amount of detectable fraudulent cow milk was 1%, and the limit of quantification was 3.1%.

UI MeSH Term Description Entries
D008892 Milk The off-white liquid secreted by the mammary glands of humans and other mammals. It contains proteins, sugar, lipids, vitamins, and minerals. Cow Milk,Cow's Milk,Milk, Cow,Milk, Cow's
D002020 Buffaloes Ruminants of the family Bovidae consisting of Bubalus arnee and Syncerus caffer. This concept is differentiated from BISON, which refers to Bison bison and Bison bonasus. Bubalus,Syncerus,Water Buffaloes,Buffalo,Water Buffalo,Buffalo, Water
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D003612 Dairying Production, storage, and distribution of DAIRY PRODUCTS. Dairy Industry,Dairy Industries,Industries, Dairy,Industry, Dairy
D005260 Female Females
D005506 Food Contamination The presence in food of harmful, unpalatable, or otherwise objectionable foreign substances, e.g. chemicals, microorganisms or diluents, before, during, or after processing or storage. Food Adulteration,Adulteration, Food,Adulterations, Food,Contamination, Food,Contaminations, Food,Food Adulterations,Food Contaminations
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity
D015415 Biomarkers Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, ENVIRONMENTAL EXPOSURE and its effects, disease diagnosis; METABOLIC PROCESSES; SUBSTANCE ABUSE; PREGNANCY; cell line development; EPIDEMIOLOGIC STUDIES; etc. Biochemical Markers,Biological Markers,Biomarker,Clinical Markers,Immunologic Markers,Laboratory Markers,Markers, Biochemical,Markers, Biological,Markers, Clinical,Markers, Immunologic,Markers, Laboratory,Markers, Serum,Markers, Surrogate,Markers, Viral,Serum Markers,Surrogate Markers,Viral Markers,Biochemical Marker,Biologic Marker,Biologic Markers,Clinical Marker,Immune Marker,Immune Markers,Immunologic Marker,Laboratory Marker,Marker, Biochemical,Marker, Biological,Marker, Clinical,Marker, Immunologic,Marker, Laboratory,Marker, Serum,Marker, Surrogate,Serum Marker,Surrogate End Point,Surrogate End Points,Surrogate Endpoint,Surrogate Endpoints,Surrogate Marker,Viral Marker,Biological Marker,End Point, Surrogate,End Points, Surrogate,Endpoint, Surrogate,Endpoints, Surrogate,Marker, Biologic,Marker, Immune,Marker, Viral,Markers, Biologic,Markers, Immune
D019075 Electrophoresis, Capillary A highly-sensitive (in the picomolar range, which is 10,000-fold more sensitive than conventional electrophoresis) and efficient technique that allows separation of PROTEINS; NUCLEIC ACIDS; and CARBOHYDRATES. (Segen, Dictionary of Modern Medicine, 1992) Capillary Zone Electrophoresis,Capillary Electrophoreses,Capillary Electrophoresis,Capillary Zone Electrophoreses,Electrophoreses, Capillary,Electrophoreses, Capillary Zone,Electrophoresis, Capillary Zone,Zone Electrophoreses, Capillary,Zone Electrophoresis, Capillary

Related Publications

Francesca Trimboli, and Nicola Costanzo, and Vincenzo Lopreiato, and Carlotta Ceniti, and Valeria M Morittu, and Antonella Spina, and Domenico Britti
October 2017, Journal of chromatography. A,
Francesca Trimboli, and Nicola Costanzo, and Vincenzo Lopreiato, and Carlotta Ceniti, and Valeria M Morittu, and Antonella Spina, and Domenico Britti
May 2017, The Journal of dairy research,
Francesca Trimboli, and Nicola Costanzo, and Vincenzo Lopreiato, and Carlotta Ceniti, and Valeria M Morittu, and Antonella Spina, and Domenico Britti
October 2014, Journal of dairy science,
Francesca Trimboli, and Nicola Costanzo, and Vincenzo Lopreiato, and Carlotta Ceniti, and Valeria M Morittu, and Antonella Spina, and Domenico Britti
January 2020, Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy,
Francesca Trimboli, and Nicola Costanzo, and Vincenzo Lopreiato, and Carlotta Ceniti, and Valeria M Morittu, and Antonella Spina, and Domenico Britti
January 1954, Nepegeszsegugy,
Francesca Trimboli, and Nicola Costanzo, and Vincenzo Lopreiato, and Carlotta Ceniti, and Valeria M Morittu, and Antonella Spina, and Domenico Britti
January 1998, Journal of capillary electrophoresis,
Francesca Trimboli, and Nicola Costanzo, and Vincenzo Lopreiato, and Carlotta Ceniti, and Valeria M Morittu, and Antonella Spina, and Domenico Britti
September 1983, Journal of dairy science,
Francesca Trimboli, and Nicola Costanzo, and Vincenzo Lopreiato, and Carlotta Ceniti, and Valeria M Morittu, and Antonella Spina, and Domenico Britti
May 2001, The Journal of dairy research,
Francesca Trimboli, and Nicola Costanzo, and Vincenzo Lopreiato, and Carlotta Ceniti, and Valeria M Morittu, and Antonella Spina, and Domenico Britti
March 2018, Food chemistry,
Francesca Trimboli, and Nicola Costanzo, and Vincenzo Lopreiato, and Carlotta Ceniti, and Valeria M Morittu, and Antonella Spina, and Domenico Britti
December 1965, Indian journal of biochemistry,
Copied contents to your clipboard!