Mitochondria Redistribution in Enterovirus A71 Infected Cells and Its Effect on Virus Replication. 2019

Yang Yang, and Haolong Cong, and Ning Du, and Xiaodong Han, and Lei Song, and Wenliang Zhang, and Chunrui Li, and Po Tien
Center for Molecular Virology, CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

Enterovirus A71 (EV-A71) is one of the main causative agents of hand, foot and mouth disease (HFMD) and it also causes severe neurologic complications in infected children. The interactions between some viruses and the host mitochondria are crucial for virus replication and pathogenicity. In this study, it was observed that EV-A71 infection resulted in a perinuclear redistribution of the mitochondria. The mitochondria rearrangement was found to require the microtubule network, the dynein complex and a low cytosolic calcium concentration. Subsequently, the EV-A71 non-structural protein 2BC was identified as the viral protein capable of inducing mitochondria clustering. The protein was found localized on mitochondria and interacted with the mitochondrial Rho GTPase 1 (RHOT1) that is a key protein required for attachment between the mitochondria and the motor proteins, which are responsible for the control of mitochondria movement. Additionally, suppressing mitochondria clustering by treating cells with nocodazole, EHNA, thapsigargin or A23187 consistently inhibited EV-A71 replication, indicating that mitochondria recruitment played a crucial role in the EV-A71 life cycle. This study identified a novel function of the EV-A71 2BC protein and provided a potential model for the regulation of mitochondrial motility in EV-A71 infection.

UI MeSH Term Description Entries
D008870 Microtubules Slender, cylindrical filaments found in the cytoskeleton of plant and animal cells. They are composed of the protein TUBULIN and are influenced by TUBULIN MODULATORS. Microtubule
D008928 Mitochondria Semiautonomous, self-reproducing organelles that occur in the cytoplasm of all cells of most, but not all, eukaryotes. Each mitochondrion is surrounded by a double limiting membrane. The inner membrane is highly invaginated, and its projections are called cristae. Mitochondria are the sites of the reactions of oxidative phosphorylation, which result in the formation of ATP. They contain distinctive RIBOSOMES, transfer RNAs (RNA, TRANSFER); AMINO ACYL T RNA SYNTHETASES; and elongation and termination factors. Mitochondria depend upon genes within the nucleus of the cells in which they reside for many essential messenger RNAs (RNA, MESSENGER). Mitochondria are believed to have arisen from aerobic bacteria that established a symbiotic relationship with primitive protoeukaryotes. (King & Stansfield, A Dictionary of Genetics, 4th ed) Mitochondrial Contraction,Mitochondrion,Contraction, Mitochondrial,Contractions, Mitochondrial,Mitochondrial Contractions
D003600 Cytosol Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components. Cytosols
D004398 Dyneins A family of multi-subunit cytoskeletal motor proteins that use the energy of ATP hydrolysis, generated by a ring of AAA ATPASES in the dynein heavy chain, to power a variety of cellular functions. Dyneins fall into two major classes based upon structural and functional criteria. ATPase, Dynein,Adenosinetriphosphatase, Dynein,Dynein,Dynein ATPase,Dynein Adenosinetriphosphatase,Dynein Heavy Chain,Dynein Intermediate Chain,Dynein Light Chain,Dynein Light Intermediate Chain,Adenosine Triphosphatase, Dynein,Dynein Heavy Chains,Dynein Intermediate Chains,Dynein Light Chains,Dynein Light Intermediate Chains,Chain, Dynein Heavy,Chain, Dynein Intermediate,Chain, Dynein Light,Chains, Dynein Heavy,Chains, Dynein Intermediate,Chains, Dynein Light,Dynein Adenosine Triphosphatase,Heavy Chain, Dynein,Heavy Chains, Dynein,Intermediate Chain, Dynein,Intermediate Chains, Dynein,Light Chain, Dynein,Light Chains, Dynein
D006367 HeLa Cells The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for, among other things, VIRUS CULTIVATION and PRECLINICAL DRUG EVALUATION assays. Cell, HeLa,Cells, HeLa,HeLa Cell
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000076662 Host Microbial Interactions Interactions between a host and microbe or microbiota. Host-Bacteria Interactions,Host-Microbe Interactions,Host-Microbial Interactions,Host-Virus Interactions,Microbe-Host Interactions,Microbial-Host Interactions,Microbiota-Host Interactions,Virus-Host Interactions,Bacteria Host Interactions,Bacterial-Host Interactions,Bacterium-Host Interactions,Host Bacteria Interactions,Host Microbe Interactions,Host Microbiota Interactions,Host Virus Interactions,Host-Fungal Interactions,Host-Microbial Interface,Microbe Host Interactions,Microbial Host Interactions,Microbiota Host Interactions,Viral-Host Interactions,Virus Host Interactions,Bacteria Host Interaction,Bacterial Host Interactions,Bacterial-Host Interaction,Bacterium Host Interactions,Bacterium-Host Interaction,Host Bacteria Interaction,Host Fungal Interactions,Host Microbe Interaction,Host Microbial Interaction,Host Microbial Interface,Host Microbiota Interaction,Host Virus Interaction,Host-Bacteria Interaction,Host-Fungal Interaction,Host-Microbe Interaction,Host-Microbial Interaction,Host-Microbial Interfaces,Host-Virus Interaction,Interaction, Host-Bacteria,Interaction, Host-Microbe,Interaction, Host-Microbial,Interaction, Host-Virus,Interaction, Microbe-Host,Interaction, Microbial-Host,Interaction, Microbiota-Host,Interaction, Virus-Host,Interactions, Host-Bacteria,Interactions, Host-Microbe,Interactions, Host-Microbial,Interactions, Host-Virus,Interactions, Microbe-Host,Interactions, Microbial-Host,Interactions, Microbiota-Host,Interactions, Virus-Host,Microbe Host Interaction,Microbe-Host Interaction,Microbial Host Interaction,Microbial-Host Interaction,Microbiota Host Interaction,Microbiota-Host Interaction,Viral Host Interactions,Viral-Host Interaction,Virus Host Interaction,Virus-Host Interaction
D014764 Viral Proteins Proteins found in any species of virus. Gene Products, Viral,Viral Gene Products,Viral Gene Proteins,Viral Protein,Protein, Viral,Proteins, Viral
D014779 Virus Replication The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle. Viral Replication,Replication, Viral,Replication, Virus,Replications, Viral,Replications, Virus,Viral Replications,Virus Replications
D017361 Viral Nonstructural Proteins Proteins encoded by a VIRAL GENOME that are not structural components of VIRUS PARTICLES. Some of these proteins may play roles within the infected cell during VIRUS REPLICATION or act in regulation of virus replication or VIRUS ASSEMBLY. Nonstructural Proteins, Viral,NS Proteins, Viral,Viral NS Proteins,Viral Non-Structural Proteins,Viral Nonstructural Protein,Viral Nonstructural Proteins NS1,Viral Nonstructural Proteins NS2,Nonstructural Protein, Viral,Viral Non Structural Proteins

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