The paper presents experimental tests of unidirectional double cantilever beams made of a glass fiber reinforced (GFRP) laminate. The critical value of the strain energy release rate (c-SERR or GIC), i.e., the mode I fracture toughness of the considered material was determined with three different methods: the compliance calibration method (CC), the modified compliance calibration method (MCC), and the corrected beam theory (CBT). Due to the common difficulties in precise definition of delamination initiation force, the Acoustic Emission (AE) technique was applied as an auxiliary source of data. The failure process was monitored, as well, in order to detect and identify different damage phenomena. This was achieved through a detailed analysis of the raw AE signal subjected to fast Fourier transformation (FFT). The frequency spectra revealed three dominating frequency bands with the basic one described by the average value of 63.1 kHz, revealing intensive delamination processes. This way, not only precise values of the critical SERR, but also the information on damage evolution during propagation of delamination, was obtained.