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A total of 694 sera have been tested in a screening program aimed at identifying monospecific reagents for HLA--DR typing. All sera were first tested on a panel of enriched B and T cells without absorption on platelets. Only sera reacting more frequently on B than on T lymphocytes were absorbed on platelets and retested on the panel. This procedure saved a considerable amount of platelets and proved to be reasonably efficient. One-hundred-and-fifty sera were found to contain an anti-B cell antibody. Significantly less frequent B cell reactivity was found when HLA--A, --B, --C antibodies could not be detected. Twenty-five sera were demonstrated to be specific for well-defined DR antigens.
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January 1985,
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July 1986,
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January 1977,
Animal blood groups and biochemical genetics,
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February 2015,
Analytical biochemistry,
P Vassalli, and
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X Chardonnens
January 2010,
Bioscience, biotechnology, and biochemistry,
