The endocytic uptake of horse-spleen ferritin by the endocardial cells in 2 bony-fish species, Xiphophorus helleri and Pollachius virens, is described. In specimens of X. helleri injected intraperitoneally by a ferritin solution 1/10 h before the sacrifice, the endocardial bristle-coated vesicles, cytoplasmic tubules, and endosomes (smooth vesicles of variable size) contain a number of ferritin particles. These particles are taken up by the bristle-coated vesicles, transferred through the cytoplasmic tubules, and emptied into the endosomes. The latter get more tightly packed by ferritin and increase in size with the time elapsed between the injection and sacrifice. After 9 h, most ferritin-packed endosomes (4 to 6 micron) contain fragments of those inclusion bodies (0.5 to 1.5 micron) which normally occur in the teleostean endocardium, and are therefore regarded as lysosomes. The ferritin-rich lysosomes increase greatly in size with time and display a width of 6 to 12 micron after 28 h. The number of cytoplasmic tubules declines rapidly with time, whereas there is a constant production of new endosomes, which probably are derived from the former. The ferritin particles are not accumulated in the endothelium of the bulbus arteriosus, whereas the hepatic endothelial cells take up some small amounts of ferritin. A similar uptake of ferritin as described above was also observed in the heart of P. virens when perfused by a ferritin solution for 3 h. The present results are discussed and compared with those previously reported for the uptake of ferritin in various tissues in fishes and mammals.(ABSTRACT TRUNCATED AT 250 WORDS)