Biomaterial Database

Aortic endothelial and smooth muscle cell interactions in culture. 1986

E Csonka, and H Jellinek

The effect of conditioned media on cell growth was studied in pig aortic smooth muscle cell conditioned medium on pig or bovine aortic endothelial cells and pig or bovine endothelial cells conditioned medium on pig aortic smooth muscle cell proliferation. Co-culturing experiments were also done in the same combinations. Cell proliferation was measured in both systems by 3H-thymidine incorporation autoradiography. In intraspecies combinations the media conditioned by confluent pig-aortic endothelial monolayer had an inhibitory effect on pig-aortic smooth muscle cell growth, but in the co-cultivation system the exponentially grown endothelial cells stimulated smooth muscle cell proliferation. The pig aortic smooth muscle cells had some stimulatory effect on pig endothelial cell proliferation in both systems. In the interspecies combination the media conditioned by confluent bovine aortic endothelial monolayer or, in the co-cultivation experiments, the bovine endothelial cells exerted a stimulatory effect on the proliferation of pig-aortic smooth muscle cells. The media conditioned by pig smooth muscle cells had also some little stimulatory effect on bovine aortic endothelial cell growth, but in the co-cultivation system the bovine aortic endothelial cells responded in a highly sensitive way to the stimulatory effect of pig aortic smooth muscle cells.

UI	MeSH Term	Description	Entries
D009131	Muscle, Smooth, Vascular	The nonstriated involuntary muscle tissue of blood vessels.	Vascular Smooth Muscle,Muscle, Vascular Smooth,Muscles, Vascular Smooth,Smooth Muscle, Vascular,Smooth Muscles, Vascular,Vascular Smooth Muscles
D002450	Cell Communication	Any of several ways in which living cells of an organism communicate with one another, whether by direct contact between cells or by means of chemical signals carried by neurotransmitter substances, hormones, and cyclic AMP.	Cell Interaction,Cell-to-Cell Interaction,Cell Communications,Cell Interactions,Cell to Cell Interaction,Cell-to-Cell Interactions,Communication, Cell,Communications, Cell,Interaction, Cell,Interaction, Cell-to-Cell,Interactions, Cell,Interactions, Cell-to-Cell
D002455	Cell Division	The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.	M Phase,Cell Division Phase,Cell Divisions,Division Phase, Cell,Division, Cell,Divisions, Cell,M Phases,Phase, Cell Division,Phase, M,Phases, M
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D003470	Culture Media	Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.	Media, Culture
D003584	Cytological Techniques	Methods used to study CELLS.	Cytologic Technics,Cytological Technic,Cytological Technics,Cytological Technique,Technic, Cytological,Technics, Cytological,Technique, Cytological,Techniques, Cytological,Cytologic Technic,Technic, Cytologic,Technics, Cytologic
D004727	Endothelium	A layer of epithelium that lines the heart, blood vessels (ENDOTHELIUM, VASCULAR), lymph vessels (ENDOTHELIUM, LYMPHATIC), and the serous cavities of the body.	Endotheliums
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001011	Aorta	The main trunk of the systemic arteries.	Aortas
D001345	Autoradiography	The making of a radiograph of an object or tissue by recording on a photographic plate the radiation emitted by radioactive material within the object. (Dorland, 27th ed)	Radioautography