Biomaterial Database

Effect of protein concentration on the binding of gold(I) to human serum albumin. 1987

S M Pedersen

The binding of aurothiosulphate, gold(I), by human serum albumin has been studied by equilibrium dialysis at four different albumin concentrations, 37 degrees, pH 7.2-7.4 and ionic strength 0.15 M. The results show that the interaction of aurothiosulphate with albumin depends on albumin concentration. This observation is linked with the previous observation that the usual independent site description cannot be used to represent the clinically important low concentration data. All the observed dependences are satisfactorily accounted for by assuming that gold(I) competes with a highly bound contaminant for the high affinity (Cys(34)-SH) site. This description is supported by the experimental observation that a fraction of this site is originally blocked both in vivo and in in vitro. The present interpretation yields a high affinity binding constant 100 times larger than found previously and provides an explanation for the lack of correlation between dose and therapeutic and toxic effects in chrysotherapy.

UI	MeSH Term	Description	Entries
D008433	Mathematics	The deductive study of shape, quantity, and dependence. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)	Mathematic
D009994	Osmolar Concentration	The concentration of osmotically active particles in solution expressed in terms of osmoles of solute per liter of solution. Osmolality is expressed in terms of osmoles of solute per kilogram of solvent.	Ionic Strength,Osmolality,Osmolarity,Concentration, Osmolar,Concentrations, Osmolar,Ionic Strengths,Osmolalities,Osmolar Concentrations,Osmolarities,Strength, Ionic,Strengths, Ionic
D011108	Polymers	Compounds formed by the joining of smaller, usually repeating, units linked by covalent bonds. These compounds often form large macromolecules (e.g., BIOPOLYMERS; PLASTICS).	Polymer
D011485	Protein Binding	The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.	Plasma Protein Binding Capacity,Binding, Protein
D006046	Gold	A yellow metallic element with the atomic symbol Au, atomic number 79, and atomic weight 197. It is used in jewelry, goldplating of other metals, as currency, and in dental restoration. Many of its clinical applications, such as ANTIRHEUMATIC AGENTS, are in the form of its salts.
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012709	Serum Albumin	A major protein in the BLOOD. It is important in maintaining the colloidal osmotic pressure and transporting large organic molecules.	Plasma Albumin,Albumin, Serum
D012971	Gold Sodium Thiosulfate	A sodium salt of gold thiosulfate. It has uses like the ORGANOGOLD COMPOUNDS.	Sodium Gold Thiosulfate,Bis(monothiosulfato(2-)-O,S)aurate(3-) Trisodium,Sanochrysin,Sanochrysine,Sanocrisin,Sodium Aurothiosulfate,Thiosulfatoaurate,Aurothiosulfate, Sodium,Gold Thiosulfate, Sodium,Sodium Thiosulfate, Gold,Thiosulfate, Gold Sodium,Thiosulfate, Sodium Gold