Biomaterial Database

Modified chromogenic alpha-amylase assay for sprouted wheat. 1979

P R Mathewson, and Y Pomeranz

Sensitivity of a test in which a commercially available substrate was used for the assay of alpha-amylase in sprouted wheat has been increased by the use of optimally buffered (pH 6.20) Cibacron-Blue dyed amylose at 60 degrees C. With the modified test, measurable alpha-amylase levels were in the range of 3--30 millidextrinizing units (mDU) as compared to the 10--90 mDU range in a test previously reported for bread wheats. The modification extends the use of the test to evaluation of sprout damage in wheats used in the manufacture of Japanese noodles and sponge cakes.

UI	MeSH Term	Description	Entries
D000516	alpha-Amylases	Enzymes that catalyze the endohydrolysis of 1,4-alpha-glycosidic linkages in STARCH; GLYCOGEN; and related POLYSACCHARIDES and OLIGOSACCHARIDES containing 3 or more 1,4-alpha-linked D-glucose units.	Taka-Amylase A,alpha-Amylase,Alpha-Amylase Bayer,Maxilase,Mégamylase,alpha-1,4-D-Glucanglucanohydrolase,Alpha Amylase Bayer,AlphaAmylase Bayer,Taka Amylase A,TakaAmylase A,alpha 1,4 D Glucanglucanohydrolase,alpha Amylase,alpha Amylases
D000681	Amylases	A group of amylolytic enzymes that cleave starch, glycogen, and related alpha-1,4-glucans. (Stedman, 25th ed) EC 3.2.1.-.	Diastase,Amylase
D013696	Temperature	The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.	Temperatures
D014908	Triticum	A plant genus of the family POACEAE that is the source of EDIBLE GRAIN. A hybrid with rye (SECALE CEREALE) is called TRITICALE. The seed is ground into FLOUR and used to make BREAD, and is the source of WHEAT GERM AGGLUTININS.	Wheat,Durum Wheat,Triticum aestivum,Triticum durum,Triticum spelta,Triticum turgidum,Triticum turgidum subsp. durum,Triticum vulgare,Durum Wheats,Wheat, Durum