-transparent.png){kind=logo}
Intravenous injection of oleic acid (OA) induces acute, edematous lung injury resembling some of the features of adult respiratory distress syndrome. One class of inflammatory agents speculated to be mediators of acute lung injury are eicosanoids. We tested the hypothesis that 5-lipoxygenase and cyclooxygenase metabolites of arachidonic acid mediate the pulmonary injury induced by OA. OA (0.1 ml), injected as a bolus into the pulmonary artery (PA) of isolated, Krebs-perfused rabbit lungs, resulted in significant (P less than .05) increases in lung weight (an index of pulmonary edema), maximum airway pressure, perfusate immunoreactive (i) 6-keto PGF1a, and a significant, though minimal, increase in perfusate i-thromboxane B2. No measurable increases were recorded in PA pressure or perfusate i-leukotriene (LT) C4/D4. Neither pretreatment with the LTD4/E4 antagonist, LY171883 (10 microM), nor the 5-lipoxygenase/cyclooxygenase inhibitor, BW755C (100 microM), attenuated the pulmonary edema. However, BW755C abrogated the increase in i6-keto PGF1a. Additionally, administration of exogenous LTD4 (100 nM) into the perfusate produced only a minimal increase in lung weight in the isolated rabbit lungs (n = 4). These results demonstrate that 5-lipoxygenase and cyclooxygenase metabolites do not appear to mediate OA-induced injury in the isolated, Krebs-perfused rabbit lung.
S A Katz, and
P V Halushka, and
W C Wise, and
J A Cook
October 1989,
The American journal of physiology,
S A Katz, and
P V Halushka, and
W C Wise, and
J A Cook
June 1981,
Journal of applied physiology: respiratory, environmental and exercise physiology,
S A Katz, and
P V Halushka, and
W C Wise, and
J A Cook
February 1998,
Journal of applied physiology (Bethesda, Md. : 1985),
S A Katz, and
P V Halushka, and
W C Wise, and
J A Cook
October 1999,
The European respiratory journal,
S A Katz, and
P V Halushka, and
W C Wise, and
J A Cook
July 2002,
Critical care medicine,
S A Katz, and
P V Halushka, and
W C Wise, and
J A Cook
January 2012,
Mediators of inflammation,
S A Katz, and
P V Halushka, and
W C Wise, and
J A Cook
April 1994,
Journal of applied physiology (Bethesda, Md. : 1985),
S A Katz, and
P V Halushka, and
W C Wise, and
J A Cook
January 1989,
Journal of cardiovascular pharmacology,
S A Katz, and
P V Halushka, and
W C Wise, and
J A Cook
April 1980,
Journal of applied physiology: respiratory, environmental and exercise physiology,
S A Katz, and
P V Halushka, and
W C Wise, and
J A Cook
July 1993,
The American review of respiratory disease,
