DL-3-n-butylphthalide Protected Retinal Müller Cells Dysfunction from Oxidative Stress. 2019

Xiaoli Xing, and Liangyu Huang, and Yingjuan Lv, and Xun Liu, and Ruihong Su, and Xiaorong Li, and Lijie Dong
Tianjin Medical University Eye Institute, Tianjin Medical University Eye Hospital, Tianjin Medical University Optometry College , Tianjin , China.

Purpose: To observe the protective effects and underlying mechanisms of dl-3-n-butylphthalide (NBP) against H2O2-induced oxidative damage in retinal Müller cells. Methods: Cultured human Müller cell line (MIO-M1line) were exposed to H2O2 for 2 hours. Cell survival was evaluated by Calcein AM cell viability assay. Dichlorofluorescein diacetate (DCFDA) + endoplasmic reticulum (ER) red fluorescent probe (ER-Tracker Red) staining was used to observe the expression level of reactive oxygen species (ROS) in ER of cells. Mitochondrial membrane potential detection (JC-1) was used to observe cell membrane potential change and early apoptosis. Cell apoptosis was detected by Hoechst33258 staining. The expressions of Nrf2, HO-1 were documented by cell Immunofluorescence staining and Western blot analysis. Results: NBP effectively improved the survival ability of Müller cells shown by MTT assay. NBP effectively alleviated the morphological and apoptotic changes induced by H2O2 stimulation by Calcein AM assay, HE staining, Hoechst 33258, JC-1 staining. H2O2 induction increased the expression level of ROS, whereas, the treatment with NBP could remarkably lower the expression level of ROS. Cell immunofluorescence staining indicated that the fluorescence staining intensity of HO-1 in the NBP group was significantly higher than that in the control group. While the western blotting results showed that the expression level of HO-1 could be increased by NBP in a time-dependent manner. The translocation of Nrf2 in nuclei was observed within 2 h and Nrf2 was identified in nuclei for up to 48 h. Conclusions: Our study demonstrated that NBP had a protective effect on H2O2-induced cytotoxicity in retinal Müller cells in vitro and that it was a potent activator of Nrf2 and HO-1signaling.

UI MeSH Term Description Entries
D008856 Microscopy, Fluorescence Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye. Fluorescence Microscopy,Immunofluorescence Microscopy,Microscopy, Immunofluorescence,Fluorescence Microscopies,Immunofluorescence Microscopies,Microscopies, Fluorescence,Microscopies, Immunofluorescence
D012164 Retinal Diseases Diseases involving the RETINA. Disease, Retinal,Diseases, Retinal,Retinal Disease
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D002470 Cell Survival The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability. Cell Viability,Cell Viabilities,Survival, Cell,Viabilities, Cell,Viability, Cell
D004721 Endoplasmic Reticulum A system of cisternae in the CYTOPLASM of many cells. In places the endoplasmic reticulum is continuous with the plasma membrane (CELL MEMBRANE) or outer membrane of the nuclear envelope. If the outer surfaces of the endoplasmic reticulum membranes are coated with ribosomes, the endoplasmic reticulum is said to be rough-surfaced (ENDOPLASMIC RETICULUM, ROUGH); otherwise it is said to be smooth-surfaced (ENDOPLASMIC RETICULUM, SMOOTH). (King & Stansfield, A Dictionary of Genetics, 4th ed) Ergastoplasm,Reticulum, Endoplasmic
D005452 Fluoresceins A family of spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one derivatives. These are used as dyes, as indicators for various metals, and as fluorescent labels in immunoassays. Tetraiodofluorescein
D005455 Fluorescent Antibody Technique Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy. Antinuclear Antibody Test, Fluorescent,Coon's Technique,Fluorescent Antinuclear Antibody Test,Fluorescent Protein Tracing,Immunofluorescence Technique,Coon's Technic,Fluorescent Antibody Technic,Immunofluorescence,Immunofluorescence Technic,Antibody Technic, Fluorescent,Antibody Technics, Fluorescent,Antibody Technique, Fluorescent,Antibody Techniques, Fluorescent,Coon Technic,Coon Technique,Coons Technic,Coons Technique,Fluorescent Antibody Technics,Fluorescent Antibody Techniques,Fluorescent Protein Tracings,Immunofluorescence Technics,Immunofluorescence Techniques,Protein Tracing, Fluorescent,Protein Tracings, Fluorescent,Technic, Coon's,Technic, Fluorescent Antibody,Technic, Immunofluorescence,Technics, Fluorescent Antibody,Technics, Immunofluorescence,Technique, Coon's,Technique, Fluorescent Antibody,Technique, Immunofluorescence,Techniques, Fluorescent Antibody,Techniques, Immunofluorescence,Tracing, Fluorescent Protein,Tracings, Fluorescent Protein
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006861 Hydrogen Peroxide A strong oxidizing agent used in aqueous solution as a ripening agent, bleach, and topical anti-infective. It is relatively unstable and solutions deteriorate over time unless stabilized by the addition of acetanilide or similar organic materials. Hydrogen Peroxide (H2O2),Hydroperoxide,Oxydol,Perhydrol,Superoxol,Peroxide, Hydrogen
D001572 Benzofurans Compounds that contain a BENZENE ring fused to a furan ring. Coumarones,Diphenylbenzofuran

Related Publications

Xiaoli Xing, and Liangyu Huang, and Yingjuan Lv, and Xun Liu, and Ruihong Su, and Xiaorong Li, and Lijie Dong
January 2022, Frontiers in pharmacology,
Xiaoli Xing, and Liangyu Huang, and Yingjuan Lv, and Xun Liu, and Ruihong Su, and Xiaorong Li, and Lijie Dong
July 2020, The Journal of international medical research,
Xiaoli Xing, and Liangyu Huang, and Yingjuan Lv, and Xun Liu, and Ruihong Su, and Xiaorong Li, and Lijie Dong
January 2018, Oxidative medicine and cellular longevity,
Xiaoli Xing, and Liangyu Huang, and Yingjuan Lv, and Xun Liu, and Ruihong Su, and Xiaorong Li, and Lijie Dong
September 2017, Experimental and therapeutic medicine,
Xiaoli Xing, and Liangyu Huang, and Yingjuan Lv, and Xun Liu, and Ruihong Su, and Xiaorong Li, and Lijie Dong
March 2021, Acta pharmacologica Sinica,
Xiaoli Xing, and Liangyu Huang, and Yingjuan Lv, and Xun Liu, and Ruihong Su, and Xiaorong Li, and Lijie Dong
May 2010, Neuroscience letters,
Xiaoli Xing, and Liangyu Huang, and Yingjuan Lv, and Xun Liu, and Ruihong Su, and Xiaorong Li, and Lijie Dong
May 2016, Investigative ophthalmology & visual science,
Xiaoli Xing, and Liangyu Huang, and Yingjuan Lv, and Xun Liu, and Ruihong Su, and Xiaorong Li, and Lijie Dong
September 2009, Brain research,
Xiaoli Xing, and Liangyu Huang, and Yingjuan Lv, and Xun Liu, and Ruihong Su, and Xiaorong Li, and Lijie Dong
May 2022, European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences,
Xiaoli Xing, and Liangyu Huang, and Yingjuan Lv, and Xun Liu, and Ruihong Su, and Xiaorong Li, and Lijie Dong
January 2016, Scientific reports,
Copied contents to your clipboard!