BIOMAT Database Logo BIOMAT Database Logo

[Kinetics of glu- and lys-plasminogen activation by the tissue activator in a fibrin clot]. 1987

E M Makogonenko, and S A Kirpa, and E V Lugovskoĭ, and N A Nazarenko, and S A Kudinov

Using a modified procedure for measuring the time of fibrin clot lysis, the kinetics of Glu- and Lys-plasminogen activation by the tissue activator was studied. Within the plasminogen concentration range of 0.4-100 nM the rate of activation of both protein forms obeys the Michaelis-Menten kinetics. At Lys-plasminogen concentration equimolar to that of fibrin, the rate of activation of the former decreases down to that of Glu-plasminogen activation. The kinetic constants for Glu- and Lys-plasminogen activation (Km) are equal to 0.055 and 0.013 microM; k = 0.19 and 0.21 s-1, respectively. The Km values for fibrin-bound Glu- and Lys-plasminogen are equal to 0.25 nM and 8 nM, respectively (k = 0.08 and 0.26 s-1, respectively). It is assumed that the tissue activator exhibits a higher affinity for the Glu-plasminogen--fibrin complex than for the Lys-plasminogen-fibrin complex.

UI MeSH Term Description Entries
D007700 Kinetics The rate dynamics in chemical or physical systems.
D010446 Peptide Fragments Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques. Peptide Fragment,Fragment, Peptide,Fragments, Peptide
D010958 Plasminogen Precursor of plasmin (FIBRINOLYSIN). It is a single-chain beta-globulin of molecular weight 80-90,000 found mostly in association with fibrinogen in plasma; plasminogen activators change it to fibrinolysin. It is used in wound debriding and has been investigated as a thrombolytic agent. Profibrinolysin,Glu-Plasminogen,Glutamic Acid 1-Plasminogen,Glutamyl Plasminogen,1-Plasminogen, Glutamic Acid,Glu Plasminogen,Glutamic Acid 1 Plasminogen,Plasminogen, Glutamyl
D010959 Tissue Plasminogen Activator A proteolytic enzyme in the serine protease family found in many tissues which converts PLASMINOGEN to FIBRINOLYSIN. It has fibrin-binding activity and is immunologically different from UROKINASE-TYPE PLASMINOGEN ACTIVATOR. The primary sequence, composed of 527 amino acids, is identical in both the naturally occurring and synthetic proteases. Alteplase,Plasminogen Activator, Tissue-Type,T-Plasminogen Activator,Tissue-Type Plasminogen Activator,Actilyse,Activase,Lysatec rt-PA,TTPA,Tisokinase,Tissue Activator D-44,Lysatec rt PA,Lysatec rtPA,Plasminogen Activator, Tissue,Plasminogen Activator, Tissue Type,T Plasminogen Activator,Tissue Activator D 44,Tissue Type Plasminogen Activator
D005337 Fibrin A protein derived from FIBRINOGEN in the presence of THROMBIN, which forms part of the blood clot. Antithrombin I
D005342 Fibrinolysis The natural enzymatic dissolution of FIBRIN. Fibrinolyses
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D066298 In Vitro Techniques Methods to study reactions or processes taking place in an artificial environment outside the living organism. In Vitro Test,In Vitro Testing,In Vitro Tests,In Vitro as Topic,In Vitro,In Vitro Technique,In Vitro Testings,Technique, In Vitro,Techniques, In Vitro,Test, In Vitro,Testing, In Vitro,Testings, In Vitro,Tests, In Vitro,Vitro Testing, In

Related Publications

E M Makogonenko, and S A Kirpa, and E V Lugovskoĭ, and N A Nazarenko, and S A Kudinov
Kinetics of the activation of plasminogen by human tissue plasminogen activator. Role of fibrin.
March 1982, The Journal of biological chemistry,
E M Makogonenko, and S A Kirpa, and E V Lugovskoĭ, and N A Nazarenko, and S A Kudinov
[Regulation with alpha-2-antiplasmin of Glu-plasminogen activation by tissue activator on fibrin].
January 2006, Ukrains'kyi biokhimichnyi zhurnal (1999 ),
E M Makogonenko, and S A Kirpa, and E V Lugovskoĭ, and N A Nazarenko, and S A Kudinov
Effects of fibrinogen and fibrin on the activation of Glu- and Lys-plasminogen by urokinase.
March 1984, Thrombosis research,
E M Makogonenko, and S A Kirpa, and E V Lugovskoĭ, and N A Nazarenko, and S A Kudinov
[The effect of kringles K1-3, K4 and K5 on lysis of fibrin clots caused by the activation of Glu- and Lys-plasminogen by a tissue activator].
January 1989, Ukrainskii biokhimicheskii zhurnal (1978),
E M Makogonenko, and S A Kirpa, and E V Lugovskoĭ, and N A Nazarenko, and S A Kudinov
Differences in effects of fibrin(ogen) fragments on the activation of 1-glu-plasminogen and 442-val-plasminogen by tissue-type plasminogen activator.
October 1983, Thrombosis research,
E M Makogonenko, and S A Kirpa, and E V Lugovskoĭ, and N A Nazarenko, and S A Kudinov
Potentiation by Lys-plasminogen of clot lysis by single or two chain urokinase-type plasminogen activator or tissue-type plasminogen activator.
June 1989, Thrombosis and haemostasis,
E M Makogonenko, and S A Kirpa, and E V Lugovskoĭ, and N A Nazarenko, and S A Kudinov
The regulation by fibrinogen and fibrin of tissue plasminogen activator kinetics and inhibition by plasminogen activator inhibitor 1.
April 2007, Journal of thrombosis and haemostasis : JTH,
E M Makogonenko, and S A Kirpa, and E V Lugovskoĭ, and N A Nazarenko, and S A Kudinov
Studies on the kinetics of plasminogen activation by tissue plasminogen activator.
June 1982, Biochimica et biophysica acta,
E M Makogonenko, and S A Kirpa, and E V Lugovskoĭ, and N A Nazarenko, and S A Kudinov
Lys- and glu-plasminogen potentiate the inhibitory effect of recombinant tissue plasminogen activator on human platelet aggregation.
June 1994, Thrombosis research,
E M Makogonenko, and S A Kirpa, and E V Lugovskoĭ, and N A Nazarenko, and S A Kudinov
Substrate inhibition of fibrin-dependent plasminogen activation by tissue-type plasminogen activator.
June 1993, The Journal of biological chemistry,
Copied contents to your clipboard!