In Vitro Culture of Embryos from LOPU-Derived Goat Oocytes. 2019

Joanna Maria G Souza-Fabjan, and Ribrio Ivan T P Batista, and Vicente José F Freitas, and Pascal Mermillod
Faculdade de Veterinária, Setor de Reprodução Animal, Universidade Federal Fluminense, Niteroi, RJ, Brazil. joannavet@gmail.com.

A high oocyte quality is the prerequisite for in vitro embryo production. Goat cumulus-oocyte complexes (COC) are mainly collected from slaughterhouse ovaries or by laparoscopic ovum pickup (LOPU) from live animals. Several features can influence the availability of good quality oocytes recovered by the LOPU technique. Interestingly, slaughterhouse and LOPU oocytes have different in vitro maturation kinetics and requirements, and thus, the IVP system must be adapted regarding the oocyte origin. Overall, the use of undefined media in the different steps makes interpretation of results more difficult, hampers their reproducibility, and introduces a sanitary risk. Thus, there is an effort worldwide to use simpler conditions for goat IVP. Although the success of IVP rates is relatively high, in vitro embryos differ from in vivo-derived ones in many aspects, resulting in lower viability. Therefore, strategies to improve in vitro embryo quality are crucial, such as the use of oviductal epithelium cells for coculture. Here we describe the main steps and culture media which can be utilized to produce embryos in vitro from LOPU or slaughterhouse oocytes in goats.

UI MeSH Term Description Entries
D009865 Oocytes Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM). Ovocytes,Oocyte,Ovocyte
D010535 Laparoscopy A procedure in which a laparoscope (LAPAROSCOPES) is inserted through a small incision near the navel to examine the abdominal and pelvic organs in the PERITONEAL CAVITY. If appropriate, biopsy or surgery can be performed during laparoscopy. Celioscopy,Laparoscopic Surgical Procedures,Peritoneoscopy,Surgical Procedures, Laparoscopic,Laparoscopic Assisted Surgery,Laparoscopic Surgery,Laparoscopic Surgical Procedure,Procedure, Laparoscopic Surgical,Procedures, Laparoscopic Surgical,Surgery, Laparoscopic,Surgical Procedure, Laparoscopic,Celioscopies,Laparoscopic Assisted Surgeries,Laparoscopic Surgeries,Laparoscopies,Peritoneoscopies,Surgeries, Laparoscopic,Surgeries, Laparoscopic Assisted,Surgery, Laparoscopic Assisted
D001755 Blastocyst A post-MORULA preimplantation mammalian embryo that develops from a 32-cell stage into a fluid-filled hollow ball of over a hundred cells. A blastocyst has two distinctive tissues. The outer layer of trophoblasts gives rise to extra-embryonic tissues. The inner cell mass gives rise to the embryonic disc and eventual embryo proper. Embryo, Preimplantation,Blastocysts,Embryos, Preimplantation,Preimplantation Embryo,Preimplantation Embryos
D005260 Female Females
D005307 Fertilization in Vitro An assisted reproductive technique that includes the direct handling and manipulation of oocytes and sperm to achieve fertilization in vitro. Test-Tube Fertilization,Fertilizations in Vitro,In Vitro Fertilization,Test-Tube Babies,Babies, Test-Tube,Baby, Test-Tube,Fertilization, Test-Tube,Fertilizations, Test-Tube,In Vitro Fertilizations,Test Tube Babies,Test Tube Fertilization,Test-Tube Baby,Test-Tube Fertilizations
D006041 Goats Any of numerous agile, hollow-horned RUMINANTS of the genus Capra, in the family Bovidae, closely related to the SHEEP. Capra,Capras,Goat
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D046149 Embryo Culture Techniques The technique of maintaining or growing mammalian EMBRYOS in vitro. This method offers an opportunity to observe EMBRYONIC DEVELOPMENT; METABOLISM; and susceptibility to TERATOGENS. Blastocyst Culture Techniques,Blastocyst Culture Technique,Culture Technique, Blastocyst,Culture Technique, Embryo,Culture Techniques, Blastocyst,Culture Techniques, Embryo,Embryo Culture Technique
D059471 In Vitro Oocyte Maturation Techniques Methods used to induce premature oocytes, that are maintained in tissue culture, to progress through developmental stages including to a stage that is competent to undergo FERTILIZATION. In Vitro Oocyte Maturation,In Vitro Maturation of Oocytes,Oocyte In Vitro Maturation

Related Publications

Joanna Maria G Souza-Fabjan, and Ribrio Ivan T P Batista, and Vicente José F Freitas, and Pascal Mermillod
August 2004, Biology of reproduction,
Joanna Maria G Souza-Fabjan, and Ribrio Ivan T P Batista, and Vicente José F Freitas, and Pascal Mermillod
March 1976, Australian journal of biological sciences,
Joanna Maria G Souza-Fabjan, and Ribrio Ivan T P Batista, and Vicente José F Freitas, and Pascal Mermillod
September 1993, Journal of animal science,
Joanna Maria G Souza-Fabjan, and Ribrio Ivan T P Batista, and Vicente José F Freitas, and Pascal Mermillod
August 2006, Fertility and sterility,
Joanna Maria G Souza-Fabjan, and Ribrio Ivan T P Batista, and Vicente José F Freitas, and Pascal Mermillod
January 1994, Theriogenology,
Joanna Maria G Souza-Fabjan, and Ribrio Ivan T P Batista, and Vicente José F Freitas, and Pascal Mermillod
December 2021, Reproduction, fertility, and development,
Joanna Maria G Souza-Fabjan, and Ribrio Ivan T P Batista, and Vicente José F Freitas, and Pascal Mermillod
May 2019, Reproduction in domestic animals = Zuchthygiene,
Joanna Maria G Souza-Fabjan, and Ribrio Ivan T P Batista, and Vicente José F Freitas, and Pascal Mermillod
April 1992, Theriogenology,
Joanna Maria G Souza-Fabjan, and Ribrio Ivan T P Batista, and Vicente José F Freitas, and Pascal Mermillod
July 1988, Journal of reproduction and fertility,
Joanna Maria G Souza-Fabjan, and Ribrio Ivan T P Batista, and Vicente José F Freitas, and Pascal Mermillod
August 2000, Fertility and sterility,
Copied contents to your clipboard!