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Growth and Purification of Vaccinia Virus Stocks for MPM Imaging. 2019
Glennys V Reynoso, and
John P Shannon, and
Jeffrey L Americo, and
James Gibbs, and
Heather D Hickman
Viral Immunity and Pathogenesis Unit, Laboratory of Clinical Immunology and Microbiology (LCIM), National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD, USA.
This chapter provides methods for the propagation, purification, and titration of vaccinia virus (VACV) and the highly attenuated strain-modified vaccinia Ankara (MVA). Additionally, we provide information on VACV recombinants we have used for intravital imaging with multiphoton excitation.
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