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On the basis of a previously developed reference system for Mycobacterium bovis, BCG, in crossed immunoelectrophoresis (CIE), antigens of Mycobacterium tuberculosis were identified in an analogous system. A majority of the numbered lines in the BCG system were also present and identified in the M. tuberculosis system. The corresponding antigens in the two systems were identified by dual dilution in CIE, and using monospecific antisera and monoclonal antibodies. Some of the antigens were specifically identified by the demonstration of enzyme activity and by means of hydroxyapatite, concanavalin A (Con A), EDTA, and blue-Sepharose. Three antigens (nos 10, 78, and 81), which were found in high concentrations in M. tuberculosis culture fluid, were not identified or were present in low concentrations in BCG culture fluid. The high percentage of corresponding antigens confirms that there is a very close taxonomic relationship between BCG and M. tuberculosis. Corresponding antigens in BCG and M. tuberculosis did not differ in electrophoretic mobility in the antigenic preparations studied.
H G Wiker, and
M Harboe, and
J Bennedsen, and
O Closs
January 1980,
Scandinavian journal of immunology,
H G Wiker, and
M Harboe, and
J Bennedsen, and
O Closs
July 1981,
The American review of respiratory disease,
H G Wiker, and
M Harboe, and
J Bennedsen, and
O Closs
November 1990,
Biulleten' eksperimental'noi biologii i meditsiny,
H G Wiker, and
M Harboe, and
J Bennedsen, and
O Closs
August 1971,
Biochemical and biophysical research communications,
H G Wiker, and
M Harboe, and
J Bennedsen, and
O Closs
August 1984,
Infection and immunity,
H G Wiker, and
M Harboe, and
J Bennedsen, and
O Closs
August 2006,
Infection and immunity,
H G Wiker, and
M Harboe, and
J Bennedsen, and
O Closs
October 1971,
The American review of respiratory disease,
H G Wiker, and
M Harboe, and
J Bennedsen, and
O Closs
November 1996,
Microbiology (Reading, England),
H G Wiker, and
M Harboe, and
J Bennedsen, and
O Closs
February 1974,
The American review of respiratory disease,
H G Wiker, and
M Harboe, and
J Bennedsen, and
O Closs
January 2018,
Infection and drug resistance,
