BIOMAT Database Logo BIOMAT Database Logo

Halothane anesthesia decreases human monocyte hydrogen peroxide generation. Protection of monocytes by activation with gamma interferon. 1987

G W Stevenson, and S Hall, and S J Rudnick, and G Alvord, and J Rossio, and W Urba, and J B Leventhal, and P Miller, and F Seleny, and H C Stevenson
Department of Anesthesia, Childrens Memorial Hospital, Northwestern University Medical School, Chicago, Illinois 60614.

In an effort to determine the impact of halothane anesthesia on certain human cell-mediated immune functions, normal, purified human monocytes and lymphocytes were exposed to halothane in vitro at varying concentrations for up to 8 hours. Subsequently, these human effector cells were analyzed for their ability to function in several cell-mediated immunologic assays. Natural killer cell activity against K-562 was unaffected by halothane in most of the donors tested. Similarly, the ability of purified monocytes to inhibit MBL-2 tumor cell growth was unchanged. Halothane appeared to decrease the proliferative response of lymphocytes to phytohemagglutinin (PHA) in approximately 50% of the normal donors tested. In contrast, the ability of monocytes to lyse antibody-coated red cell targets (ADCC) was unaffected by even maximal exposure to halothane. Of interest was the finding that human monocytes exposed to as low as 2% halothane anesthesia for 4 hours displayed a dramatic down-regulation of hydrogen peroxide (H2O2) release. Since it is known that hydrogen peroxide and other incompletely reduced forms of oxygen secreted by monocytes can play a major role in the antimicrobial, antitumor, and inflammatory functions of these cells, this finding may help explain the enhanced susceptibility of post-operative patients to infections.

UI MeSH Term Description Entries
D007165 Immunosuppression Therapy Deliberate prevention or diminution of the host's immune response. It may be nonspecific as in the administration of immunosuppressive agents (drugs or radiation) or by lymphocyte depletion or may be specific as in desensitization or the simultaneous administration of antigen and immunosuppressive drugs. Antirejection Therapy,Immunosuppression,Immunosuppressive Therapy,Anti-Rejection Therapy,Therapy, Anti-Rejection,Therapy, Antirejection,Anti Rejection Therapy,Anti-Rejection Therapies,Antirejection Therapies,Immunosuppression Therapies,Immunosuppressions,Immunosuppressive Therapies,Therapies, Immunosuppression,Therapies, Immunosuppressive,Therapy, Immunosuppression,Therapy, Immunosuppressive
D007371 Interferon-gamma The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES. Interferon Type II,Interferon, Immune,gamma-Interferon,Interferon, gamma,Type II Interferon,Immune Interferon,Interferon, Type II
D007694 Killer Cells, Natural Bone marrow-derived lymphocytes that possess cytotoxic properties, classically directed against transformed and virus-infected cells. Unlike T CELLS; and B CELLS; NK CELLS are not antigen specific. The cytotoxicity of natural killer cells is determined by the collective signaling of an array of inhibitory and stimulatory CELL SURFACE RECEPTORS. A subset of T-LYMPHOCYTES referred to as NATURAL KILLER T CELLS shares some of the properties of this cell type. NK Cells,Natural Killer Cells,Cell, NK,Cell, Natural Killer,Cells, NK,Cells, Natural Killer,Killer Cell, Natural,NK Cell,Natural Killer Cell
D008213 Lymphocyte Activation Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION. Blast Transformation,Blastogenesis,Lymphoblast Transformation,Lymphocyte Stimulation,Lymphocyte Transformation,Transformation, Blast,Transformation, Lymphoblast,Transformation, Lymphocyte,Activation, Lymphocyte,Stimulation, Lymphocyte
D009000 Monocytes Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles. Monocyte
D010835 Phytohemagglutinins Mucoproteins isolated from the kidney bean (Phaseolus vulgaris); some of them are mitogenic to lymphocytes, others agglutinate all or certain types of erythrocytes or lymphocytes. They are used mainly in the study of immune mechanisms and in cell culture. Kidney Bean Lectin,Kidney Bean Lectins,Lectins, Kidney Bean,Phaseolus vulgaris Lectin,Phaseolus vulgaris Lectins,Phytohemagglutinin,Hemagglutinins, Plant,Lectin, Kidney Bean,Lectin, Phaseolus vulgaris,Lectins, Phaseolus vulgaris,Plant Hemagglutinins
D003602 Cytotoxicity, Immunologic The phenomenon of target cell destruction by immunologically active effector cells. It may be brought about directly by sensitized T-lymphocytes or by lymphoid or myeloid "killer" cells, or it may be mediated by cytotoxic antibody, cytotoxic factor released by lymphoid cells, or complement. Tumoricidal Activity, Immunologic,Immunologic Cytotoxicity,Immunologic Tumoricidal Activities,Immunologic Tumoricidal Activity,Tumoricidal Activities, Immunologic
D006221 Halothane A nonflammable, halogenated, hydrocarbon anesthetic that provides relatively rapid induction with little or no excitement. Analgesia may not be adequate. NITROUS OXIDE is often given concomitantly. Because halothane may not produce sufficient muscle relaxation, supplemental neuromuscular blocking agents may be required. (From AMA Drug Evaluations Annual, 1994, p178) 1,1,1-Trifluoro-2-Chloro-2-Bromoethane,Fluothane,Ftorotan,Narcotan
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006861 Hydrogen Peroxide A strong oxidizing agent used in aqueous solution as a ripening agent, bleach, and topical anti-infective. It is relatively unstable and solutions deteriorate over time unless stabilized by the addition of acetanilide or similar organic materials. Hydrogen Peroxide (H2O2),Hydroperoxide,Oxydol,Perhydrol,Superoxol,Peroxide, Hydrogen

Related Publications

G W Stevenson, and S Hall, and S J Rudnick, and G Alvord, and J Rossio, and W Urba, and J B Leventhal, and P Miller, and F Seleny, and H C Stevenson
Activation of redox-systems of monocytes by hydrogen peroxide.
January 2006, BioFactors (Oxford, England),
G W Stevenson, and S Hall, and S J Rudnick, and G Alvord, and J Rossio, and W Urba, and J B Leventhal, and P Miller, and F Seleny, and H C Stevenson
Pulmonary surfactant inhibits activation of human monocytes by recombinant interferon-gamma.
July 1994, Immunology,
G W Stevenson, and S Hall, and S J Rudnick, and G Alvord, and J Rossio, and W Urba, and J B Leventhal, and P Miller, and F Seleny, and H C Stevenson
Interferon-gamma Stimulates Monocyte Chemotactic Protein-1 Expression by Monocytes.
January 1994, Mediators of inflammation,
G W Stevenson, and S Hall, and S J Rudnick, and G Alvord, and J Rossio, and W Urba, and J B Leventhal, and P Miller, and F Seleny, and H C Stevenson
Administration of recombinant interferon gamma to cancer patients enhances monocyte secretion of hydrogen peroxide.
December 1985, Proceedings of the National Academy of Sciences of the United States of America,
G W Stevenson, and S Hall, and S J Rudnick, and G Alvord, and J Rossio, and W Urba, and J B Leventhal, and P Miller, and F Seleny, and H C Stevenson
Induction of interferon-gamma production by human natural killer cells stimulated by hydrogen peroxide.
April 1985, Journal of immunology (Baltimore, Md. : 1950),
G W Stevenson, and S Hall, and S J Rudnick, and G Alvord, and J Rossio, and W Urba, and J B Leventhal, and P Miller, and F Seleny, and H C Stevenson
Lymphokine-mediated activation of human monocytes: neutralization by monoclonal antibody to interferon-gamma.
April 1984, Cellular immunology,
G W Stevenson, and S Hall, and S J Rudnick, and G Alvord, and J Rossio, and W Urba, and J B Leventhal, and P Miller, and F Seleny, and H C Stevenson
Gamma-interferon-induced activation of latent transforming growth factor-beta by human monocytes.
January 1990, Annals of the New York Academy of Sciences,
G W Stevenson, and S Hall, and S J Rudnick, and G Alvord, and J Rossio, and W Urba, and J B Leventhal, and P Miller, and F Seleny, and H C Stevenson
Immunosuppression by D-penicillamine in vitro. Inhibition of human T lymphocyte proliferation by copper- or ceruloplasmin-dependent generation of hydrogen peroxide and protection by monocytes.
January 1984, The Journal of clinical investigation,
G W Stevenson, and S Hall, and S J Rudnick, and G Alvord, and J Rossio, and W Urba, and J B Leventhal, and P Miller, and F Seleny, and H C Stevenson
Effect of recombinant interferon-gamma on hydrogen peroxide-releasing capacity of monocyte-derived macrophages from patients with lepromatous leprosy.
August 1986, Journal of immunology (Baltimore, Md. : 1950),
G W Stevenson, and S Hall, and S J Rudnick, and G Alvord, and J Rossio, and W Urba, and J B Leventhal, and P Miller, and F Seleny, and H C Stevenson
The interferon-gamma receptor on human monocytes, monocyte-like cell lines and polymorphonuclear leucocytes.
April 1990, Biochemical Society transactions,
Copied contents to your clipboard!