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Bovine FSH (bFSH) was used to immunize BALB/c mice. Spleen cells were fused to the SP 2/0 cell line to produce hybridomas that secreted monoclonal antibodies to bFSH. One of these antibodies (USDA-bFSH-MC28) was extensively characterized and found to be a gamma 1 with kappa light chains, having extremely low cross-reactivity with other bovine pituitary hormones and with ovine and porcine FSH. The dissociation constant as measured by Scatchard analysis was 4.3 nmol/l, and proved to be in a very useful range for affinity chromatography. In an essentially one-step immunoaffinity chromatography procedure, bFSH was easily isolated in a single chromatographic step from crude anterior pituitary homogenate with better yield and with the same purity as classical chromatographic techniques.
K F Miller, and
R A Goldsby, and
D J Bolt
November 2004,
General and comparative endocrinology,
K F Miller, and
R A Goldsby, and
D J Bolt
January 1996,
Preparative biochemistry & biotechnology,
K F Miller, and
R A Goldsby, and
D J Bolt
January 1993,
Archivum immunologiae et therapiae experimentalis,
K F Miller, and
R A Goldsby, and
D J Bolt
January 2008,
Journal of AOAC International,
K F Miller, and
R A Goldsby, and
D J Bolt
May 2001,
Current protocols in molecular biology,
K F Miller, and
R A Goldsby, and
D J Bolt
February 1987,
Journal of immunological methods,
K F Miller, and
R A Goldsby, and
D J Bolt
September 1987,
Journal of neurochemistry,
K F Miller, and
R A Goldsby, and
D J Bolt
January 2009,
Methods in enzymology,
K F Miller, and
R A Goldsby, and
D J Bolt
May 2001,
Biochimica et biophysica acta,
K F Miller, and
R A Goldsby, and
D J Bolt
December 1991,
Kisaengch'unghak chapchi. The Korean journal of parasitology,
