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Functional and molecular probes are described which are useful to identify a 3-methylcholanthrene-inducible phenol UDP-glucuronosyltransferase (GTMC) from rat liver. Two different procedures for isolation of GTMC were compared, method 1 utilizing DEAE-Sepharose chromatography or method 2, chromatofocusing. Method 2 appeared to be superior in separating different isoenzymes. Subsequently the enzyme was purified by affinity chromatography on UDP-hexanolamine Sepharose. With both methods a protein was purified with a subunit Mr of 55,000, catalyzing glucuronidation of a variety of planar phenols and, in particular, of benzo(a)pyrene-3,6-quinol to its mono- and diglucuronide. Antibodies to GTMC recognized a polypeptide with a subunit Mr of 55,000 as the major 3-methylcholanthrene-inducible isoenzyme in rat liver microsomes. The described functional and molecular probes may help to differentiate GTMC from similar isoenzymes conjugating planar phenols and to elucidate its regulation and biological function.
K W Bock, and
G Schirmer, and
M D Green, and
T R Tephly
March 1997,
Pharmacology & toxicology,
K W Bock, and
G Schirmer, and
M D Green, and
T R Tephly
October 1992,
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K W Bock, and
G Schirmer, and
M D Green, and
T R Tephly
November 1986,
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K W Bock, and
G Schirmer, and
M D Green, and
T R Tephly
April 1994,
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K W Bock, and
G Schirmer, and
M D Green, and
T R Tephly
July 1991,
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K W Bock, and
G Schirmer, and
M D Green, and
T R Tephly
February 1977,
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K W Bock, and
G Schirmer, and
M D Green, and
T R Tephly
December 1989,
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K W Bock, and
G Schirmer, and
M D Green, and
T R Tephly
June 1990,
Cancer research,
K W Bock, and
G Schirmer, and
M D Green, and
T R Tephly
May 1986,
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K W Bock, and
G Schirmer, and
M D Green, and
T R Tephly
July 1979,
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