Biomaterial Database

[Synthesis of group-specific polysaccharide by different strains of Neisseria meningitidis serogroup B under various conditions]. 1988

L D Sivtseva, and L A Levina

Sonicated lysates of 5 N. meningitidis strains, serogroup B, obtained from two solid serum-free culture media (a medium with casamino acids and a medium with Hottinger's hydrolysate) were studied with the aim of comparing the capacity of different group B meningococcal strains for the accumulation of group-specific polysaccharide. In the lysates obtained after 7-hour growth no sialic acid was found. After 20-hour cultivation, group-specific polysaccharide was detected in the lysates obtained from 4 out of 5 strains. All sonicated lysates obtained in these experiments were serologically active. The lysates obtained from the medium containing casamino acid had a higher content of group-specific polysaccharide. N. meningitidis strain 125, obtained at the Mechnikov Central Research Institute for Vaccines and Sera (Moscow) by selection, showed the highest content of capsular polysaccharide in microbial cells and the stable yield of biomass.

UI	MeSH Term	Description	Entries
D007120	Immunochemistry	Field of chemistry that pertains to immunological phenomena and the study of chemical reactions related to antigen stimulation of tissues. It includes physicochemical interactions between antigens and antibodies.
D009345	Neisseria meningitidis	A species of gram-negative, aerobic BACTERIA. It is a commensal and pathogen only of humans, and can be carried asymptomatically in the NASOPHARYNX. When found in cerebrospinal fluid it is the causative agent of cerebrospinal meningitis (MENINGITIS, MENINGOCOCCAL). It is also found in venereal discharges and blood. There are at least 13 serogroups based on antigenic differences in the capsular polysaccharides; the ones causing most meningitis infections being A, B, C, Y, and W-135. Each serogroup can be further classified by serotype, serosubtype, and immunotype.	Diplokokkus intracellularis meningitidis,Meningococcus,Micrococcus intracellularis,Micrococcus meningitidis,Micrococcus meningitidis cerebrospinalis,Neisseria weichselbaumii
D011135	Polysaccharides, Bacterial	Polysaccharides found in bacteria and in capsules thereof.	Bacterial Polysaccharides
D003470	Culture Media	Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.	Media, Culture
D012703	Serotyping	Process of determining and distinguishing species of bacteria or viruses based on antigens they share.	Serotypings
D013045	Species Specificity	The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.	Species Specificities,Specificities, Species,Specificity, Species
D013997	Time Factors	Elements of limited time intervals, contributing to particular results or situations.	Time Series,Factor, Time,Time Factor