BIOMAT Database Logo BIOMAT Database Logo

Differences in immune responses of pigs vaccinated with Salmonella Typhimurium and S. Choleraesuis strains and challenged with S. Choleraesuis. 2019

Yung-Fu Chang, and Jiun-Nub Hou, and Hsin-Hung Lin, and Chean-Ping Wu, and Chishih Chu
Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, 602 Tower Rd., Ithaca, NY, 14853, United States.

S. Choleraesuis (Choleraesuis) and S. Typhimurium (Typhimurium) cause salmonellosis in pigs and humans. The effects of vaccine strains pSV-less Typhimurium OU5048 and Choleraesuis OU7266 and SPI-2-mutant Choleraesuis SC2284 on the immune responses of pigs against Typhimurium, Choleraesuis, and S. Enteritidis (Enteritidis) with or without the virulence plasmid (pSV) were determined. After oral vaccination of three vaccine groups and challenge with Choleraesuis CN36, the level of Salmonella-specific IgG in sera and the bactericidal effects and superoxide generation of peripheral blood mononuclear cells (PBMCs) and polymorphonuclear leukocytes (PMNs) against the above strains were determined using ELISA and NBT assay, respectively. Among three vaccine strains tested, OU7266 stimulated the highest Salmonella-specific IgG levels. Complement inactivation increased IgG concentration, while E. coli absorption reduced IgG levels. The pSV-containing strains were less resistant to serum killing than the pSV-less strains, and Enteritidis exhibited the lowest resistance to serum killing. Serovars tested, vaccine strains, and timeline periods postvaccination and challenge were important factors affecting superoxide production. The two Choleraesuis vaccine strains stimulated greater levels of superoxide from PMNs and PBMCs than the Typhimurium strains. The PMNs and PBMCs in challenged and vaccinated pigs reduced more superoxide than those in challenged hosts. In vaccinated hosts, pSV-less Salmonella strains triggered lower levels of PMN/PBMC-generated superoxide upon challenge than strains with pSV against Enteritidis and Choleraesuis. Overall, Choleraesuis OU7266 may be better than the other vaccine strains in generating the greatest IgG levels, serum bactericidal activity and superoxide levels. The pSV likely influences the immune responses.

UI MeSH Term Description Entries
D007074 Immunoglobulin G The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B. Gamma Globulin, 7S,IgG,IgG Antibody,Allerglobuline,IgG(T),IgG1,IgG2,IgG2A,IgG2B,IgG3,IgG4,Immunoglobulin GT,Polyglobin,7S Gamma Globulin,Antibody, IgG,GT, Immunoglobulin
D007963 Leukocytes, Mononuclear Mature LYMPHOCYTES and MONOCYTES transported by the blood to the body's extravascular space. They are morphologically distinguishable from mature granulocytic leukocytes by their large, non-lobed nuclei and lack of coarse, heavily stained cytoplasmic granules. Mononuclear Leukocyte,Mononuclear Leukocytes,PBMC Peripheral Blood Mononuclear Cells,Peripheral Blood Human Mononuclear Cells,Peripheral Blood Mononuclear Cell,Peripheral Blood Mononuclear Cells,Leukocyte, Mononuclear
D009504 Neutrophils Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes. LE Cells,Leukocytes, Polymorphonuclear,Polymorphonuclear Leukocytes,Polymorphonuclear Neutrophils,Neutrophil Band Cells,Band Cell, Neutrophil,Cell, LE,LE Cell,Leukocyte, Polymorphonuclear,Neutrophil,Neutrophil Band Cell,Neutrophil, Polymorphonuclear,Polymorphonuclear Leukocyte,Polymorphonuclear Neutrophil
D003165 Complement System Proteins Serum glycoproteins participating in the host defense mechanism of COMPLEMENT ACTIVATION that creates the COMPLEMENT MEMBRANE ATTACK COMPLEX. Included are glycoproteins in the various pathways of complement activation (CLASSICAL COMPLEMENT PATHWAY; ALTERNATIVE COMPLEMENT PATHWAY; and LECTIN COMPLEMENT PATHWAY). Complement Proteins,Complement,Complement Protein,Hemolytic Complement,Complement, Hemolytic,Protein, Complement,Proteins, Complement,Proteins, Complement System
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D005260 Female Females
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000907 Antibodies, Bacterial Immunoglobulins produced in a response to BACTERIAL ANTIGENS. Bacterial Antibodies
D012475 Salmonella A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria that utilizes citrate as a sole carbon source. It is pathogenic for humans, causing enteric fevers, gastroenteritis, and bacteremia. Food poisoning is the most common clinical manifestation. Organisms within this genus are separated on the basis of antigenic characteristics, sugar fermentation patterns, and bacteriophage susceptibility.
D012481 Salmonella Infections, Animal Infections in animals with bacteria of the genus SALMONELLA. Animal Salmonella Infection,Animal Salmonella Infections,Infection, Animal Salmonella,Infections, Animal Salmonella,Salmonella Infection, Animal

Related Publications

Yung-Fu Chang, and Jiun-Nub Hou, and Hsin-Hung Lin, and Chean-Ping Wu, and Chishih Chu
Acute phase responses of pigs challenged orally with Salmonella typhimurium.
July 2000, Journal of animal science,
Yung-Fu Chang, and Jiun-Nub Hou, and Hsin-Hung Lin, and Chean-Ping Wu, and Chishih Chu
Pathogenicity differences of Salmonella enterica serovars Typhimurium, Enteritidis, and Choleraesuis-specific virulence plasmids and clinical S. Choleraesuis strains with large plasmids to the human THP-1 cell death.
March 2019, Microbial pathogenesis,
Yung-Fu Chang, and Jiun-Nub Hou, and Hsin-Hung Lin, and Chean-Ping Wu, and Chishih Chu
Experimental vaccination of pigs with avirulent rough strains of Salmonella choleraesuis.
November 1965, The British veterinary journal,
Yung-Fu Chang, and Jiun-Nub Hou, and Hsin-Hung Lin, and Chean-Ping Wu, and Chishih Chu
Ciprofloxacin-resistant Salmonella enterica Typhimurium and Choleraesuis from pigs to humans, Taiwan.
January 2004, Emerging infectious diseases,
Yung-Fu Chang, and Jiun-Nub Hou, and Hsin-Hung Lin, and Chean-Ping Wu, and Chishih Chu
Studies on the pathogenesis of Salmonella typhimurium and Salmonella choleraesuis var kunzendorf infection in weanling pigs.
January 1986, American journal of veterinary research,
Yung-Fu Chang, and Jiun-Nub Hou, and Hsin-Hung Lin, and Chean-Ping Wu, and Chishih Chu
Effects of Ascophyllum nodosum extract on growth performance and immune function of young pigs challenged with Salmonella typhimurium.
July 2002, Journal of animal science,
Yung-Fu Chang, and Jiun-Nub Hou, and Hsin-Hung Lin, and Chean-Ping Wu, and Chishih Chu
Oxygenated drinking water enhances immune activity in pigs and increases immune responses of pigs during Salmonella typhimurium infection.
December 2012, The Journal of veterinary medical science,
Yung-Fu Chang, and Jiun-Nub Hou, and Hsin-Hung Lin, and Chean-Ping Wu, and Chishih Chu
Effect of flavophospholipol on fecal microbiota in weaned pigs challenged with Salmonella Typhimurium.
January 2020, Porcine health management,
Yung-Fu Chang, and Jiun-Nub Hou, and Hsin-Hung Lin, and Chean-Ping Wu, and Chishih Chu
Protection and immune responses induced by attenuated Salmonella typhimurium UK-1 strains.
March 1999, Microbial pathogenesis,
Yung-Fu Chang, and Jiun-Nub Hou, and Hsin-Hung Lin, and Chean-Ping Wu, and Chishih Chu
The immunity produced by a rough Salmonella dublin variant against Salmonella typhimurium and Salmonella choleraesuis infection guinea-pigs.
September 1966, The Journal of hygiene,
Copied contents to your clipboard!