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The contact sites of yeast tRNA1Val with tRNA(adenine-1-)-methyltransferase (EC 2.1.1.36) were studied by comparing the partial digests of free and enzyme bound tRNA. The RNAases Sa, V1, S1 and A were used for this purpose. Phosphodiester bonds on the proximal end of the acceptor stem and adjacent D- and T psi-stems, forming a continuous area, are protected by the methylase from the action of RNAases. On the contrary an enhancement of phosphodiester bond splitting of the D- and T psi-loops and of the anticodon stem is observed in the presence of methylase, which is interpreted as tertiary structure relaxation of tRNA owing to complex formation. The isotherm of ethidium bromide adsorption on free and methylase bound tRNA has shown that in enzyme shields approximately 50% of tRNA double stranded regions.
A S Gambarian, and
I A Morozov, and
T V Venkstern, and
N V Kuznetsova, and
O F Borisova
June 1994,
Bioscience, biotechnology, and biochemistry,
A S Gambarian, and
I A Morozov, and
T V Venkstern, and
N V Kuznetsova, and
O F Borisova
January 2000,
Nucleic acids symposium series,
A S Gambarian, and
I A Morozov, and
T V Venkstern, and
N V Kuznetsova, and
O F Borisova
January 1988,
Molekuliarnaia biologiia,
A S Gambarian, and
I A Morozov, and
T V Venkstern, and
N V Kuznetsova, and
O F Borisova
October 1987,
Bioorganicheskaia khimiia,
A S Gambarian, and
I A Morozov, and
T V Venkstern, and
N V Kuznetsova, and
O F Borisova
September 1993,
Nucleic acids research,
A S Gambarian, and
I A Morozov, and
T V Venkstern, and
N V Kuznetsova, and
O F Borisova
August 2006,
Proteins,
A S Gambarian, and
I A Morozov, and
T V Venkstern, and
N V Kuznetsova, and
O F Borisova
December 1987,
Journal of molecular biology,
A S Gambarian, and
I A Morozov, and
T V Venkstern, and
N V Kuznetsova, and
O F Borisova
January 1983,
Nucleic acids symposium series,
A S Gambarian, and
I A Morozov, and
T V Venkstern, and
N V Kuznetsova, and
O F Borisova
April 1987,
Bioorganicheskaia khimiia,
A S Gambarian, and
I A Morozov, and
T V Venkstern, and
N V Kuznetsova, and
O F Borisova
June 1993,
Journal of molecular biology,
