BIOMAT Database Logo BIOMAT Database Logo

Serum albumin adducts in the molecular epidemiology of aflatoxin carcinogenesis: correlation with aflatoxin B1 intake and urinary excretion of aflatoxin M1. 1988

L S Gan, and P L Skipper, and X C Peng, and J D Groopman, and J S Chen, and G N Wogan, and S R Tannenbaum
Department of Applied Biological Sciences, Massachusetts Institute of Technology, Cambridge 02178.

Aflatoxin-serum albumin adducts in the blood of 42 residents of Guangxi Province, People's Republic of China, were determined and compared with intake of aflatoxin B1 (AFB1) and excretion of aflatoxin M1 (AFM1) in urine. Blood specimens were obtained during the same period that urine was collected and that diet was sampled. Serum albumin was isolated from blood by affinity chromatography on Reactive Blue 2-Sepharose and subjected to enzymatic proteolysis using Pronase. Immunoreactive products were purified by immunoaffinity chromatography and quantified by competitive radioimmunoassay. A highly significant correlation (r = 0.60, P less than 0.00003) of adduct level with AFM1 excretion was observed. An equally highly significant correlation of adduct level with intake (r = 0.69, P less than 0.000001) was also observed. From the slope of the regression line for adduct level as a function of intake, it was calculated that 1.4-2.3% of ingested AFB1 becomes covalently bound to serum albumin, a value very similar to that observed when rats are administered AFB1.

UI MeSH Term Description Entries
D002681 China A country spanning from central Asia to the Pacific Ocean. Inner Mongolia,Manchuria,People's Republic of China,Sinkiang,Mainland China
D004305 Dose-Response Relationship, Drug The relationship between the dose of an administered drug and the response of the organism to the drug. Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000348 Aflatoxins Furano-furano-benzopyrans that are produced by ASPERGILLUS from STERIGMATOCYSTIN. They are structurally related to COUMARINS and easily oxidized to an epoxide form to become ALKYLATING AGENTS. Members of the group include AFLATOXIN B1; aflatoxin B2, aflatoxin G1, aflatoxin G2; AFLATOXIN M1; and aflatoxin M2. Aflatoxin
D012709 Serum Albumin A major protein in the BLOOD. It is important in maintaining the colloidal osmotic pressure and transporting large organic molecules. Plasma Albumin,Albumin, Serum
D016604 Aflatoxin B1 A potent hepatotoxic and hepatocarcinogenic mycotoxin produced by the Aspergillus flavus group of fungi. It is also mutagenic, teratogenic, and causes immunosuppression in animals. It is found as a contaminant in peanuts, cottonseed meal, corn, and other grains. The mycotoxin requires epoxidation to aflatoxin B1 2,3-oxide for activation. Microsomal monooxygenases biotransform the toxin to the less toxic metabolites aflatoxin M1 and Q1. Aflatoxin B(1),Aflatoxin B,Aflatoxin B1 Dihydrochloride, (6aR-cis)-Isomer,Aflatoxin B1, (6aR-cis)-Isomer, 14C-Labeled,Aflatoxin B1, (6aR-cis)-Isomer, 2H-Labeled,Aflatoxin B1, (6aR-cis)-Isomer, 3H-Labeled,Aflatoxin B1, cis(+,-)-Isomer,HSDB-3453,NSC-529592,HSDB 3453,HSDB3453,NSC 529592,NSC529592
D016607 Aflatoxin M1 A 4-hydroxylated metabolite of AFLATOXIN B1, one of the MYCOTOXINS from ASPERGILLUS tainted food. It is associated with LIVER damage and cancer resulting from its P450 activation to the epoxide which alkylates DNA. Toxicity depends on the balance of liver enzymes that activate it (CYTOCHROME P-450) and others that detoxify it (GLUTATHIONE S TRANSFERASE) (Pharmac Ther 50.443 1991). Primates & rat are sensitive while mouse and hamster are tolerant (Canc Res 29.236 1969). Aflatoxin M(1),4-Hydroxyaflatoxin B1,Aflatoxin M,Aflatoxin M1, cis(+-)-Isomer,4 Hydroxyaflatoxin B1

Related Publications

L S Gan, and P L Skipper, and X C Peng, and J D Groopman, and J S Chen, and G N Wogan, and S R Tannenbaum
Correlation of dietary aflatoxin B1 levels with excretion of aflatoxin M1 in human urine.
April 1987, Cancer research,
L S Gan, and P L Skipper, and X C Peng, and J D Groopman, and J S Chen, and G N Wogan, and S R Tannenbaum
Urinary excretion of aflatoxin B1 and its metabolite M1 in growing swine fed with aflatoxin contaminated diet.
May 1980, Taiwan yi xue hui za zhi. Journal of the Formosan Medical Association,
L S Gan, and P L Skipper, and X C Peng, and J D Groopman, and J S Chen, and G N Wogan, and S R Tannenbaum
Comparison of urinary aflatoxin M1 and aflatoxin albumin adducts as biomarkers for assessing aflatoxin exposure in Tanzanian children.
March 2018, Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals,
L S Gan, and P L Skipper, and X C Peng, and J D Groopman, and J S Chen, and G N Wogan, and S R Tannenbaum
Aflatoxin B1 albumin adducts in plasma and aflatoxin M1 in urine are associated with plasma concentrations of vitamins A and E.
December 2010, International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vitaminologie et de nutrition,
L S Gan, and P L Skipper, and X C Peng, and J D Groopman, and J S Chen, and G N Wogan, and S R Tannenbaum
Urinary excretion of aflatoxin M1 after administration of aflatoxin B1 in sucrose- or starch-rich diets.
September 1978, The British journal of nutrition,
L S Gan, and P L Skipper, and X C Peng, and J D Groopman, and J S Chen, and G N Wogan, and S R Tannenbaum
The binding of aflatoxin B1 with serum albumin.
January 1973, Biochemical pharmacology,
L S Gan, and P L Skipper, and X C Peng, and J D Groopman, and J S Chen, and G N Wogan, and S R Tannenbaum
Detectable levels of serum aflatoxin B1-albumin adducts in the United Kingdom population: implications for aflatoxin-B1 exposure in the United Kingdom.
May 1998, Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology,
L S Gan, and P L Skipper, and X C Peng, and J D Groopman, and J S Chen, and G N Wogan, and S R Tannenbaum
The Effects of Aflatoxin B1 Intake in Assaf Dairy Ewes on Aflatoxin M1 Excretion, Milk Yield, Haematology and Biochemical Profile.
January 2023, Animals : an open access journal from MDPI,
L S Gan, and P L Skipper, and X C Peng, and J D Groopman, and J S Chen, and G N Wogan, and S R Tannenbaum
Molecular dosimetry of hepatic aflatoxin B1-DNA adducts: linear correlation with hepatic cancer risk.
August 1989, Regulatory toxicology and pharmacology : RTP,
L S Gan, and P L Skipper, and X C Peng, and J D Groopman, and J S Chen, and G N Wogan, and S R Tannenbaum
Molecular dosimetry of urinary aflatoxin-N7-guanine and serum aflatoxin-albumin adducts predicts chemoprotection by 1,2-dithiole-3-thione in rats.
January 1992, Carcinogenesis,
Copied contents to your clipboard!