-transparent.png){kind=logo}
Isolated human pancreatic islets converted [3H8]arachidonate to compounds with the high-performance liquid-chromatographic mobility of cyclooxygenase products, including prostaglandin E2 (PGE2), PGF2 alpha, and the lipoxygenase product 12-HETE. Human islet synthesis of PGE2, PGF2 alpha, and 12-HETE from endogenous arachidonate was demonstrated with stable isotope dilution-gas chromatographic-negative ion-chemical ionization-mass spectrometric analysis. Pharmacologic inhibition of arachidonate metabolism by both lipoxygenase and cyclooxygenase pathways with BW 755C strongly suppressed glucose-induced insulin secretion from perifused human islets, and the selective cyclooxygenase inhibitor indomethacin enhanced insulin secretion. These findings are similar to those reported for islets isolated from rats and suggest that arachidonate metabolites may modulate glucose-induced insulin secretion in humans.
J Turk, and
J H Hughes, and
R A Easom, and
B A Wolf, and
D W Scharp, and
P E Lacy, and
M L McDaniel
March 1985,
Biochimica et biophysica acta,
J Turk, and
J H Hughes, and
R A Easom, and
B A Wolf, and
D W Scharp, and
P E Lacy, and
M L McDaniel
June 1994,
Diabetes,
J Turk, and
J H Hughes, and
R A Easom, and
B A Wolf, and
D W Scharp, and
P E Lacy, and
M L McDaniel
March 1976,
The Journal of clinical investigation,
J Turk, and
J H Hughes, and
R A Easom, and
B A Wolf, and
D W Scharp, and
P E Lacy, and
M L McDaniel
November 1998,
Pancreas,
J Turk, and
J H Hughes, and
R A Easom, and
B A Wolf, and
D W Scharp, and
P E Lacy, and
M L McDaniel
January 2002,
The Journal of clinical endocrinology and metabolism,
J Turk, and
J H Hughes, and
R A Easom, and
B A Wolf, and
D W Scharp, and
P E Lacy, and
M L McDaniel
June 1984,
Biochimica et biophysica acta,
J Turk, and
J H Hughes, and
R A Easom, and
B A Wolf, and
D W Scharp, and
P E Lacy, and
M L McDaniel
September 1969,
Acta diabetologica latina,
J Turk, and
J H Hughes, and
R A Easom, and
B A Wolf, and
D W Scharp, and
P E Lacy, and
M L McDaniel
March 2018,
Journal of diabetes investigation,
J Turk, and
J H Hughes, and
R A Easom, and
B A Wolf, and
D W Scharp, and
P E Lacy, and
M L McDaniel
February 1972,
FEBS letters,
J Turk, and
J H Hughes, and
R A Easom, and
B A Wolf, and
D W Scharp, and
P E Lacy, and
M L McDaniel
September 1976,
Acta endocrinologica,
