OBJECTIVE The purpose of this study was to confirm that the difference in excess post-exercise oxygen consumption (EPOC) during exercise of the spending the same calories between the continuous and interval exercise. METHODS Thirty-four healthy college students who did not regularly exercise volunteered to participate in our study. Continuous exercise was performed on an ergometer for 30 min at 60% of maximal oxygen consumption (VO2 max). Interval exercise was performed on a cycle ergometer at 80% VO2 max for 2 min initially, followed by 40% VO2 max for 1 min, and 80% VO2 max for 3 min. This was repeated six times for a total of 26 min. RESULTS The major findings were as follows: (1) energy consumption during exercise was not significantly different between continuous exercise and interval exercise groups; (2) EPOC was higher in interval exercise than in continuous exercise for all dependent variables (i.e., total oxygen consumption, total calories, summation of heart rate); and (3) there were no significant differences in the lipid profile between continuous and interval groups. CONCLUSIONS Our study confirmed that after equalizing energy expenditure for continuous and interval exercise on a cycle ergometer in subjects in their twenties, interval exercise results in higher EPOC than continuous exercise. These data suggest that interval exercise may be more effective than continuous exercise in reducing body fat, for a given amount of energy expenditure.