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The differential expression of genes for the PSE-4 beta-lactamase in Pseudomonas aeruginosa and the Enterobacteriaceae. 1988

A J Reid, and I N Simpson, and P B Harper, and S G Amyes
Department of Bacteriology, Medical School, University of Edinburgh, Scotland.

The PSE-4 beta-lactamase has been identified, for the first time, in two non-pseudomonal strains. The gene in Klebsiella pneumoniae 241 (dal1) was located on a plasmid (pUK700) and was freely transferable to other enterobacterial strains and Pseudomonas aeruginosa. On the other hand, the gene in Enterobacter cloacae A113 (dal2) could only be transferred in the presence of a mobilizing plasmid. When both these genes were transferred within the Enterobacteriaceae, the beta-lactamase produced was slightly different from the prototype 'Dalgleish' PSE-4 enzyme. However, when dal1 and dal2 were transferred to P. aeruginosa the enzyme expressed was identical to this prototype enzyme. In addition, both these genes expressed higher levels of PSE-4 beta-lactamase production in P. aeruginosa than found in the Enterobacteriaceae. Thus it appears that the biochemical properties of the PSE-4 gene products from dal1 and dal2 are host-modified.

UI MeSH Term Description Entries
D007525 Isoelectric Focusing Electrophoresis in which a pH gradient is established in a gel medium and proteins migrate until they reach the site (or focus) at which the pH is equal to their isoelectric point. Electrofocusing,Focusing, Isoelectric
D007700 Kinetics The rate dynamics in chemical or physical systems.
D008970 Molecular Weight The sum of the weight of all the atoms in a molecule. Molecular Weights,Weight, Molecular,Weights, Molecular
D010957 Plasmids Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS. Episomes,Episome,Plasmid
D011550 Pseudomonas aeruginosa A species of gram-negative, aerobic, rod-shaped bacteria commonly isolated from clinical specimens (wound, burn, and urinary tract infections). It is also found widely distributed in soil and water. P. aeruginosa is a major agent of nosocomial infection. Bacillus aeruginosus,Bacillus pyocyaneus,Bacterium aeruginosum,Bacterium pyocyaneum,Micrococcus pyocyaneus,Pseudomonas polycolor,Pseudomonas pyocyanea
D004755 Enterobacteriaceae A family of gram-negative, facultatively anaerobic, rod-shaped bacteria that do not form endospores. Its organisms are distributed worldwide with some being saprophytes and others being plant and animal parasites. Many species are of considerable economic importance due to their pathogenic effects on agriculture and livestock. Coliform Bacilli,Enterobacteria,Ewingella,Leclercia,Paracolobactrum,Sodalis
D005786 Gene Expression Regulation Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation. Gene Action Regulation,Regulation of Gene Expression,Expression Regulation, Gene,Regulation, Gene Action,Regulation, Gene Expression
D005798 Genes, Bacterial The functional hereditary units of BACTERIA. Bacterial Gene,Bacterial Genes,Gene, Bacterial
D001618 beta-Lactamases Enzymes found in many bacteria which catalyze the hydrolysis of the amide bond in the beta-lactam ring. Well known antibiotics destroyed by these enzymes are penicillins and cephalosporins. beta-Lactamase,beta Lactamase,beta Lactamases
D013379 Substrate Specificity A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts. Specificities, Substrate,Specificity, Substrate,Substrate Specificities

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