The production and radioimmunoassay application of monoclonal antibodies to fluorescein isothiocyanate (FITC). 1988

B Micheel, and P Jantscheff, and V Böttger, and G Scharte, and G Kaiser, and P Stolley, and L Karawajew
Central Institute of Molecular Biology, Academy of Sciences of the G.D.R., Berlin-Buch.

Monoclonal antibodies (MoAbs) were produced against the fluorescence marker fluorescein isothiocyanate (FITC). FITC was used as a hapten to label different proteins and the anti-FITC MoAbs were used to identify these labelled proteins in a solid-phase radioimmunoassay and in cellular radioimmuno-binding assays for the demonstration of antigens and antibodies.

UI MeSH Term Description Entries
D007202 Indicators and Reagents Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499) Indicator,Reagent,Reagents,Indicators,Reagents and Indicators
D008807 Mice, Inbred BALB C An inbred strain of mouse that is widely used in IMMUNOLOGY studies and cancer research. BALB C Mice, Inbred,BALB C Mouse, Inbred,Inbred BALB C Mice,Inbred BALB C Mouse,Mice, BALB C,Mouse, BALB C,Mouse, Inbred BALB C,BALB C Mice,BALB C Mouse
D011863 Radioimmunoassay Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation. Radioimmunoassays
D002459 Cell Fusion Fusion of somatic cells in vitro or in vivo, which results in somatic cell hybridization. Cell Fusions,Fusion, Cell,Fusions, Cell
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D003110 Colonic Neoplasms Tumors or cancer of the COLON. Cancer of Colon,Colon Adenocarcinoma,Colon Cancer,Cancer of the Colon,Colon Neoplasms,Colonic Cancer,Neoplasms, Colonic,Adenocarcinoma, Colon,Adenocarcinomas, Colon,Cancer, Colon,Cancer, Colonic,Cancers, Colon,Cancers, Colonic,Colon Adenocarcinomas,Colon Cancers,Colon Neoplasm,Colonic Cancers,Colonic Neoplasm,Neoplasm, Colon,Neoplasm, Colonic,Neoplasms, Colon
D005452 Fluoresceins A family of spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one derivatives. These are used as dyes, as indicators for various metals, and as fluorescent labels in immunoassays. Tetraiodofluorescein
D006063 Chorionic Gonadotropin A gonadotropic glycoprotein hormone produced primarily by the PLACENTA. Similar to the pituitary LUTEINIZING HORMONE in structure and function, chorionic gonadotropin is involved in maintaining the CORPUS LUTEUM during pregnancy. CG consists of two noncovalently linked subunits, alpha and beta. Within a species, the alpha subunit is virtually identical to the alpha subunits of the three pituitary glycoprotein hormones (TSH, LH, and FSH), but the beta subunit is unique and confers its biological specificity (CHORIONIC GONADOTROPIN, BETA SUBUNIT, HUMAN). Chorionic Gonadotropin, Human,HCG (Human Chorionic Gonadotropin),Biogonadil,Choriogonadotropin,Choriogonin,Chorulon,Gonabion,Human Chorionic Gonadotropin,Pregnyl,Gonadotropin, Chorionic,Gonadotropin, Human Chorionic
D006241 Haptens Small antigenic determinants capable of eliciting an immune response only when coupled to a carrier. Haptens bind to antibodies but by themselves cannot elicit an antibody response. Hapten,Contact-Sensitizing Agents,Agents, Contact-Sensitizing,Contact Sensitizing Agents
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

Related Publications

B Micheel, and P Jantscheff, and V Böttger, and G Scharte, and G Kaiser, and P Stolley, and L Karawajew
June 1988, Journal of immunological methods,
B Micheel, and P Jantscheff, and V Böttger, and G Scharte, and G Kaiser, and P Stolley, and L Karawajew
January 1983, Microscopica acta,
B Micheel, and P Jantscheff, and V Böttger, and G Scharte, and G Kaiser, and P Stolley, and L Karawajew
August 1985, The Journal of investigative dermatology,
B Micheel, and P Jantscheff, and V Böttger, and G Scharte, and G Kaiser, and P Stolley, and L Karawajew
January 1973, Journal of immunological methods,
B Micheel, and P Jantscheff, and V Böttger, and G Scharte, and G Kaiser, and P Stolley, and L Karawajew
January 1965, Journal of hygiene, epidemiology, microbiology, and immunology,
B Micheel, and P Jantscheff, and V Böttger, and G Scharte, and G Kaiser, and P Stolley, and L Karawajew
May 1987, Zhonghua zhong liu za zhi [Chinese journal of oncology],
B Micheel, and P Jantscheff, and V Böttger, and G Scharte, and G Kaiser, and P Stolley, and L Karawajew
January 1989, Advances in biotechnological processes,
B Micheel, and P Jantscheff, and V Böttger, and G Scharte, and G Kaiser, and P Stolley, and L Karawajew
January 1973, Journal of immunological methods,
B Micheel, and P Jantscheff, and V Böttger, and G Scharte, and G Kaiser, and P Stolley, and L Karawajew
January 1983, The EMBO journal,
B Micheel, and P Jantscheff, and V Böttger, and G Scharte, and G Kaiser, and P Stolley, and L Karawajew
February 2007, Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology,
Copied contents to your clipboard!