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Wheat germ agglutinin (WGA) is low mitogenic or nonmitogenic for human T lymphocytes and inhibits phytohemagglutinin (PHA)-induced mitotic response of the lymphocytes. In this study, the effect of WGA was analyzed in terms of interleukin 2 (IL2) production, expression of IL2 receptor, and IL2 responsiveness of the T lymphocytes. WGA as well as PHA could induce IL2 mRNA and IL2 production and also elevate cytoplasmic free Ca2+ concentration. The IL2 production was reduced by inhibitors of calmodulin and protein kinase C. The IL2 receptor (Tac) expression was induced at about 20% of the lymphocytes by WGA and the expression induced by PHA was not blocked by the addition of WGA. The lymphocytes precultured with WGA for 3 days could proliferate by the addition of IL2 after removal of WGA. The IL2-dependent proliferation of PHA-blasts was blocked by the addition of WGA. These results indicate that WGA inhibits T lymphocyte proliferation by inhibiting the responsiveness of the lymphocytes to IL2 but not by interfering with IL2 production and IL2 receptor expression.
K Kawakami, and
Y Yamamoto, and
K Onoue
January 1985,
Journal of immunology (Baltimore, Md. : 1950),
K Kawakami, and
Y Yamamoto, and
K Onoue
September 1987,
Veterinary immunology and immunopathology,
K Kawakami, and
Y Yamamoto, and
K Onoue
September 1982,
Proceedings of the National Academy of Sciences of the United States of America,
K Kawakami, and
Y Yamamoto, and
K Onoue
February 1980,
FEBS letters,
K Kawakami, and
Y Yamamoto, and
K Onoue
November 1976,
The Journal of experimental medicine,
K Kawakami, and
Y Yamamoto, and
K Onoue
April 1985,
FEBS letters,
K Kawakami, and
Y Yamamoto, and
K Onoue
May 1987,
Journal of immunology (Baltimore, Md. : 1950),
K Kawakami, and
Y Yamamoto, and
K Onoue
October 1986,
Biochimica et biophysica acta,
K Kawakami, and
Y Yamamoto, and
K Onoue
October 1983,
Cellular immunology,
K Kawakami, and
Y Yamamoto, and
K Onoue
October 1985,
Biochemical and biophysical research communications,
