BIOMAT Database Logo BIOMAT Database Logo

High-Throughput LC-MS/MS Method for Chiral Amino Acid Analysis Without Derivatization. 2019

Yosuke Nakano, and Moyu Taniguchi, and Yutaka Umakoshi, and Daisuke Watai, and Eiichiro Fukusaki
Department of Biotechnology, Graduate School of Engineering, Osaka University, Osaka, Japan.

D-Amino acids have recently attracted much attention in various research fields including medical, clinical, and food industry due to their important biological functions that differ from L-amino acid. Most chiral amino acid separation techniques require complicated derivatization procedures in order to achieve the desirable chromatographic behavior and detectability. This chapter describes a highly sensitive analytical method for the enantioseparation of chiral amino acids without any derivatization process using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The method allows the simultaneous analysis of 18 D-amino acids with high sensitivity and reproducibility. Additionally, this chapter also focuses on the application of the method to real samples for the quantification of targeted amino acids.

UI MeSH Term Description Entries
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D000596 Amino Acids Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins. Amino Acid,Acid, Amino,Acids, Amino
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity
D013237 Stereoisomerism The phenomenon whereby compounds whose molecules have the same number and kind of atoms and the same atomic arrangement, but differ in their spatial relationships. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed) Molecular Stereochemistry,Stereoisomers,Stereochemistry, Molecular,Stereoisomer
D015203 Reproducibility of Results The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results. Reliability and Validity,Reliability of Result,Reproducibility Of Result,Reproducibility of Finding,Validity of Result,Validity of Results,Face Validity,Reliability (Epidemiology),Reliability of Results,Reproducibility of Findings,Test-Retest Reliability,Validity (Epidemiology),Finding Reproducibilities,Finding Reproducibility,Of Result, Reproducibility,Of Results, Reproducibility,Reliabilities, Test-Retest,Reliability, Test-Retest,Result Reliabilities,Result Reliability,Result Validities,Result Validity,Result, Reproducibility Of,Results, Reproducibility Of,Test Retest Reliability,Validity and Reliability,Validity, Face
D053719 Tandem Mass Spectrometry A mass spectrometry technique using two (MS/MS) or more mass analyzers. With two in tandem, the precursor ions are mass-selected by a first mass analyzer, and focused into a collision region where they are then fragmented into product ions which are then characterized by a second mass analyzer. A variety of techniques are used to separate the compounds, ionize them, and introduce them to the first mass analyzer. For example, for in GC-MS/MS, GAS CHROMATOGRAPHY-MASS SPECTROMETRY is involved in separating relatively small compounds by GAS CHROMATOGRAPHY prior to injecting them into an ionization chamber for the mass selection. Mass Spectrometry-Mass Spectrometry,Mass Spectrometry Mass Spectrometry,Mass Spectrometry, Tandem
D057166 High-Throughput Screening Assays Rapid methods of measuring the effects of an agent in a biological or chemical assay. The assay usually involves some form of automation or a way to conduct multiple assays at the same time using sample arrays. High-Throughput Screening,High-Throughput Biological Assays,High-Throughput Chemical Assays,High-Throughput Screening Methods,Assay, High-Throughput Biological,Assay, High-Throughput Chemical,Assay, High-Throughput Screening,Biological Assay, High-Throughput,Chemical Assay, High-Throughput,High Throughput Biological Assays,High Throughput Chemical Assays,High Throughput Screening,High Throughput Screening Assays,High Throughput Screening Methods,High-Throughput Biological Assay,High-Throughput Chemical Assay,High-Throughput Screening Assay,High-Throughput Screening Method,High-Throughput Screenings,Screening Assay, High-Throughput,Screening Method, High-Throughput,Screening, High-Throughput

Related Publications

Yosuke Nakano, and Moyu Taniguchi, and Yutaka Umakoshi, and Daisuke Watai, and Eiichiro Fukusaki
Amino acid analysis via LC-MS method after derivatization with quaternary phosphonium.
January 2012, Methods in molecular biology (Clifton, N.J.),
Yosuke Nakano, and Moyu Taniguchi, and Yutaka Umakoshi, and Daisuke Watai, and Eiichiro Fukusaki
Derivatization-based High-throughput Bioanalysis by LC-MS.
January 2017, Analytical sciences : the international journal of the Japan Society for Analytical Chemistry,
Yosuke Nakano, and Moyu Taniguchi, and Yutaka Umakoshi, and Daisuke Watai, and Eiichiro Fukusaki
A Practical Method for Amino Acid Analysis by LC-MS Using Precolumn Derivatization with Urea.
April 2023, International journal of molecular sciences,
Yosuke Nakano, and Moyu Taniguchi, and Yutaka Umakoshi, and Daisuke Watai, and Eiichiro Fukusaki
Analysis of amino acids without derivatization in barley extracts by LC-MS-MS.
August 2008, Analytical and bioanalytical chemistry,
Yosuke Nakano, and Moyu Taniguchi, and Yutaka Umakoshi, and Daisuke Watai, and Eiichiro Fukusaki
Derivatization-based quadruplex LC/ESI-MS/MS method for high throughput quantification of serum dehydroepiandrosterone sulfate.
April 2021, Biomedical chromatography : BMC,
Yosuke Nakano, and Moyu Taniguchi, and Yutaka Umakoshi, and Daisuke Watai, and Eiichiro Fukusaki
LC-MS/MS Method for High-Throughput Analysis of Methylmalonic Acid in Serum, Plasma, and Urine: Method for Analyzing Isomers Without Chromatographic Separation.
January 2022, Methods in molecular biology (Clifton, N.J.),
Yosuke Nakano, and Moyu Taniguchi, and Yutaka Umakoshi, and Daisuke Watai, and Eiichiro Fukusaki
High-Sensitivity Micro LC-MS/MS Assay for Serum Estradiol without Derivatization.
July 2016, The journal of applied laboratory medicine,
Yosuke Nakano, and Moyu Taniguchi, and Yutaka Umakoshi, and Daisuke Watai, and Eiichiro Fukusaki
High-Throughput Analysis of Methylmalonic Acid in Serum, Plasma, and Urine by LC-MS/MS. Method for Analyzing Isomers Without Chromatographic Separation.
January 2016, Methods in molecular biology (Clifton, N.J.),
Yosuke Nakano, and Moyu Taniguchi, and Yutaka Umakoshi, and Daisuke Watai, and Eiichiro Fukusaki
Comparison of amino acid derivatization reagents for LC-ESI-MS analysis. Introducing a novel phosphazene-based derivatization reagent.
September 2012, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
Yosuke Nakano, and Moyu Taniguchi, and Yutaka Umakoshi, and Daisuke Watai, and Eiichiro Fukusaki
Coumarin-based derivatization reagent for LC-MS analysis of amino acids.
January 2023, Talanta,
Copied contents to your clipboard!