Biomaterial Database

Surface chemistry of gold nanoparticles determines interactions with bovine serum albumin. 2019

Gongke Wang, and Changling Yan, and Shuyan Gao, and Yufang Liu

School of Chemistry and Chemical Engineering, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, Henan Normal University, Xinxiang, Henan 453007, PR China. Electronic address: 031145@htu.cn.

Protein coronas provide a novel technique for the bio identification of nanoparticles in physiological environments, to further elucidate the biological effects of nanoparticles in biomedical applications. Herein, we investigated the adsorption of bovine serum albumin (BSA) on gold nanoparticles (AuNPs) with different surface modifications (citrate, cysteine, polyethylene glycol (PEG), and cetyltrimethylammonium bromide (CTAB)) using UV-vis absorption spectroscopy, fluorescence spectroscopy, circular dichroism (CD), Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), and transmission electron microscopy (TEM) techniques. It was revealed that the binding of AuNPs modified with citrate, cysteine, PEG (2k), and CTAB to BSA, appeared to be of the static quenching type, with binding constants in the range of from 108 to 1010 M-1. We also found that the conformation of BSA underwent various changes upon association with the different AuNP surface modifications. In addition, the preliminary results indicated that the thicknesses of protein coronas and the aggregation behaviors of AuNPs were closely related to their surface properties. These findings offered important insights into the essence of the interactions between nanoparticles and proteins toward the development of safe and effective nanomaterials in biological systems.

UI	MeSH Term	Description	Entries
D002417	Cattle	Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.	Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D006046	Gold	A yellow metallic element with the atomic symbol Au, atomic number 79, and atomic weight 197. It is used in jewelry, goldplating of other metals, as currency, and in dental restoration. Many of its clinical applications, such as ANTIRHEUMATIC AGENTS, are in the form of its salts.
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012710	Serum Albumin, Bovine	Serum albumin from cows, commonly used in in vitro biological studies. (From Stedman, 25th ed)	Fetal Bovine Serum,Fetal Calf Serum,Albumin Bovine,Bovine Albumin,Bovine Serum Albumin,Albumin, Bovine,Albumin, Bovine Serum,Bovine Serum, Fetal,Bovine, Albumin,Calf Serum, Fetal,Serum, Fetal Bovine,Serum, Fetal Calf
D013499	Surface Properties	Characteristics or attributes of the outer boundaries of objects, including molecules.	Properties, Surface,Property, Surface,Surface Property
D053768	Metal Nanoparticles	Nanoparticles produced from metals whose uses include biosensors, optics, and catalysts. In biomedical applications the particles frequently involve the noble metals, especially gold and silver.	Metal Nanocrystals,Metallic Nanocrystals,Metallic Nanoparticles,Metal Nanocrystal,Metal Nanoparticle,Metallic Nanocrystal,Metallic Nanoparticle,Nanocrystal, Metal,Nanocrystal, Metallic,Nanocrystals, Metal,Nanocrystals, Metallic,Nanoparticle, Metal,Nanoparticle, Metallic,Nanoparticles, Metal,Nanoparticles, Metallic