Biomaterial Database

[Isolation and identification of endophytic fungi producing harpagoside and harpagide from Scrophularia ningpoensis]. 2019

Zhan-Yun Shen, and Bo Zhu, and Quan-Long Zhang, and Lu-Ping Qin

College of Pharmaceutical Sciences,Zhejiang Chinese Medical University Hangzhou 311402,China College of Traditional Chinese Medicine,Zhejiang Pharmaceutical College Ningbo 315100,China.

The endophytic fungi from root,main stem,branch and leaf of Scrophularia ningpoensis were isolated from Zhejiang,whether these strains could yield harpagide or harpagoside were tested by HPLC and LC-MS. According to the morphological characteristic and the similarity of the nucleotide sequence of internal transcribed spacer( ITS) between r DNAs,the strains producing harpagide or harpagoside were identified. The results showed that 210 strains were isolated from the samples,which were classified into 9 orders,13 families and 17 genera by morphological study. Harpagide was detected in endogenous fungi ZJ17 and harpagoside was detected in endogenous fungi ZJ25 by HPLC coupled with LC-MS. ZJ17 was identified as Alternaria alternate and ZJ25 was identified as A.gaisen by its morphology and authenticated by ITS( ITS4 and ITS5 regions and the intervening 5. 8 S rDNA region).

UI	MeSH Term	Description	Entries
D011714	Pyrans		Pyran
D002681	China	A country spanning from central Asia to the Pacific Ocean.	Inner Mongolia,Manchuria,People's Republic of China,Sinkiang,Mainland China
D004271	DNA, Fungal	Deoxyribonucleic acid that makes up the genetic material of fungi.	Fungal DNA
D005658	Fungi	A kingdom of eukaryotic, heterotrophic organisms that live parasitically as saprobes, including MUSHROOMS; YEASTS; smuts, molds, etc. They reproduce either sexually or asexually, and have life cycles that range from simple to complex. Filamentous fungi, commonly known as molds, refer to those that grow as multicellular colonies.	Fungi, Filamentous,Molds,Filamentous Fungi,Filamentous Fungus,Fungus,Fungus, Filamentous,Mold
D006027	Glycosides	Any compound that contains a constituent sugar, in which the hydroxyl group attached to the first carbon is substituted by an alcoholic, phenolic, or other group. They are named specifically for the sugar contained, such as glucoside (glucose), pentoside (pentose), fructoside (fructose), etc. Upon hydrolysis, a sugar and nonsugar component (aglycone) are formed. (From Dorland, 28th ed; From Miall's Dictionary of Chemistry, 5th ed)	Glycoside
D057889	Iridoid Glycosides	A subclass of iridoid compounds that include a glycoside moiety, usually found at the C-1 position.	Iridoidglycosides,Secoiridoid Glycosides,Glycosides, Iridoid,Glycosides, Secoiridoid
D060026	Endophytes	An endosymbiont that is either a bacterium or fungus living part of its life in a plant. Endophytes can benefit host plants by preventing pathogenic organisms from colonizing them.	Endophyte,Endophytic Organism
D021903	DNA, Ribosomal Spacer	The intergenic DNA segments that are between the ribosomal RNA genes (internal transcribed spacers) and between the tandemly repeated units of rDNA (external transcribed spacers and nontranscribed spacers).	Ribosomal Spacer DNA,Spacer DNA, Ribosomal
D032266	Scrophularia	A plant genus of the family SCROPHULARIACEAE. Members contain iridoids.	Figwort Betony,Betonies, Figwort,Betony, Figwort,Figwort Betonies,Scrophularias