Vinculin, talin, and integrins are localized at specific adhesion sites of malignant B lymphocytes. 1988

P C Marchisio, and L Bergui, and G C Corbascio, and O Cremona, and N D'Urso, and M Schena, and L Tesio, and F Caligaris-Cappio
Dipartimento di Scienze Biomediche e Oncologia Umana, Università di Torino, Italy.

The microanatomy of the dot-shaped, close-contact sites called podosomes and the mechanism of their formation have been investigated in vitro in the malignant lymphocytes of B chronic lymphocytic leukemia (B-CLL). In this paper the authors demonstrate that in B-CLL podosomes: (1) vinculin, talin, and beta 2 integrin (CD18) rings surround an F-actin core; (2) the beta 1 integrin is localized within the F-actin core; (3) the beta 3 integrin is not present. This distribution and organization of adhesion-related molecules appears to be unique to B-CLL lymphocytes, since it has not been observed in normal B cells. B-CLL adhesion and podosome formation are inhibited by the synthetic peptide GRGDSP that contains the Arg-Gly-Asp (RGD) sequence.

UI MeSH Term Description Entries
D007945 Leukemia, Lymphoid Leukemia associated with HYPERPLASIA of the lymphoid tissues and increased numbers of circulating malignant LYMPHOCYTES and lymphoblasts. Leukemia, Lymphocytic,Lymphocytic Leukemia,Lymphoid Leukemia,Leukemias, Lymphocytic,Leukemias, Lymphoid,Lymphocytic Leukemias,Lymphoid Leukemias
D008562 Membrane Glycoproteins Glycoproteins found on the membrane or surface of cells. Cell Surface Glycoproteins,Surface Glycoproteins,Cell Surface Glycoprotein,Membrane Glycoprotein,Surface Glycoprotein,Glycoprotein, Cell Surface,Glycoprotein, Membrane,Glycoprotein, Surface,Glycoproteins, Cell Surface,Glycoproteins, Membrane,Glycoproteins, Surface,Surface Glycoprotein, Cell,Surface Glycoproteins, Cell
D009124 Muscle Proteins The protein constituents of muscle, the major ones being ACTINS and MYOSINS. More than a dozen accessory proteins exist including TROPONIN; TROPOMYOSIN; and DYSTROPHIN. Muscle Protein,Protein, Muscle,Proteins, Muscle
D002448 Cell Adhesion Adherence of cells to surfaces or to other cells. Adhesion, Cell,Adhesions, Cell,Cell Adhesions
D003598 Cytoskeletal Proteins Major constituent of the cytoskeleton found in the cytoplasm of eukaryotic cells. They form a flexible framework for the cell, provide attachment points for organelles and formed bodies, and make communication between parts of the cell possible. Proteins, Cytoskeletal
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D001402 B-Lymphocytes Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation. B-Cells, Lymphocyte,B-Lymphocyte,Bursa-Dependent Lymphocytes,B Cells, Lymphocyte,B Lymphocyte,B Lymphocytes,B-Cell, Lymphocyte,Bursa Dependent Lymphocytes,Bursa-Dependent Lymphocyte,Lymphocyte B-Cell,Lymphocyte B-Cells,Lymphocyte, Bursa-Dependent,Lymphocytes, Bursa-Dependent
D016023 Integrins A family of transmembrane glycoproteins (MEMBRANE GLYCOPROTEINS) consisting of noncovalent heterodimers. They interact with a wide variety of ligands including EXTRACELLULAR MATRIX PROTEINS; COMPLEMENT, and other cells, while their intracellular domains interact with the CYTOSKELETON. The integrins consist of at least three identified families: the cytoadhesin receptors (RECEPTORS, CYTOADHESIN), the leukocyte adhesion receptors (RECEPTORS, LEUKOCYTE ADHESION), and the VERY LATE ANTIGEN RECEPTORS. Each family contains a common beta-subunit (INTEGRIN BETA CHAINS) combined with one or more distinct alpha-subunits (INTEGRIN ALPHA CHAINS). These receptors participate in cell-matrix and cell-cell adhesion in many physiologically important processes, including embryological development; HEMOSTASIS; THROMBOSIS; WOUND HEALING; immune and nonimmune defense mechanisms; and oncogenic transformation. Integrin
D016596 Vinculin A cytoskeletal protein associated with cell-cell and cell-matrix interactions. The amino acid sequence of human vinculin has been determined. The protein consists of 1066 amino acid residues and its gene has been assigned to chromosome 10. alpha-Vinculin,beta-Vinculin,Isovinculin,alpha Vinculin,beta Vinculin

Related Publications

P C Marchisio, and L Bergui, and G C Corbascio, and O Cremona, and N D'Urso, and M Schena, and L Tesio, and F Caligaris-Cappio
March 2006, The Journal of biological chemistry,
P C Marchisio, and L Bergui, and G C Corbascio, and O Cremona, and N D'Urso, and M Schena, and L Tesio, and F Caligaris-Cappio
January 1990, Cell motility and the cytoskeleton,
P C Marchisio, and L Bergui, and G C Corbascio, and O Cremona, and N D'Urso, and M Schena, and L Tesio, and F Caligaris-Cappio
November 2004, Biochemical Society transactions,
P C Marchisio, and L Bergui, and G C Corbascio, and O Cremona, and N D'Urso, and M Schena, and L Tesio, and F Caligaris-Cappio
March 2013, The Journal of biological chemistry,
P C Marchisio, and L Bergui, and G C Corbascio, and O Cremona, and N D'Urso, and M Schena, and L Tesio, and F Caligaris-Cappio
July 2004, Nature,
P C Marchisio, and L Bergui, and G C Corbascio, and O Cremona, and N D'Urso, and M Schena, and L Tesio, and F Caligaris-Cappio
February 1996, Journal of muscle research and cell motility,
P C Marchisio, and L Bergui, and G C Corbascio, and O Cremona, and N D'Urso, and M Schena, and L Tesio, and F Caligaris-Cappio
January 1995, Nature,
P C Marchisio, and L Bergui, and G C Corbascio, and O Cremona, and N D'Urso, and M Schena, and L Tesio, and F Caligaris-Cappio
December 2007, The Journal of cell biology,
P C Marchisio, and L Bergui, and G C Corbascio, and O Cremona, and N D'Urso, and M Schena, and L Tesio, and F Caligaris-Cappio
February 2016, The Journal of biological chemistry,
P C Marchisio, and L Bergui, and G C Corbascio, and O Cremona, and N D'Urso, and M Schena, and L Tesio, and F Caligaris-Cappio
February 1992, Biochemical Society transactions,
Copied contents to your clipboard!