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Evaluation of three commercial systems for the identification of pathogenic Neisseria and Branhamella species against the conventional method. 1988

C Durussel, and H H Siegrist
Institut de microbiologie, CHUV, Lausanne, Switzerland.

We compared three commercial systems for the identification of Neisseria and Branhamella spp. with the conventional method using cystine-tryptic digest agar (CTA) supplemented with carbohydrates, DNase production, and nitrate reduction. We evaluated the API quadFerm+ [( API], Analytab Products, Inc., Plainview, N.Y.), NEISSERIA [( Pasteur], Diagnostics Pasteur, Marnes-la-Coquette, France), and Neisseria Identification Discs [( Oxoid], Oxoid Ltd., Basingstoke, England) using the conventional method as a reference. One hundred and twenty-nine strains were included in this study. The conventional method identified 125 strains (96.9%). Four strains of N. gonorrhoeae remained glucose-negative in the CTA media but gave positive reactions in the API system. API, Pasteur, and Oxoid identified 126 (97.7%), 124 (96.1%), and 62 strains (48.1%), respectively. API and Pasteur seem to be very useful systems for the differentiation of clinically significant species of Neisseria and Branhamella. API has the additional advantage of requiring only a 3 h incubation period.

UI MeSH Term Description Entries
D009343 Neisseria A genus of gram-negative, aerobic, coccoid bacteria whose organisms are part of the normal flora of the oropharynx, nasopharynx, and genitourinary tract. Some species are primary pathogens for humans.
D011237 Predictive Value of Tests In screening and diagnostic tests, the probability that a person with a positive test is a true positive (i.e., has the disease), is referred to as the predictive value of a positive test; whereas, the predictive value of a negative test is the probability that the person with a negative test does not have the disease. Predictive value is related to the sensitivity and specificity of the test. Negative Predictive Value,Positive Predictive Value,Predictive Value Of Test,Predictive Values Of Tests,Negative Predictive Values,Positive Predictive Values,Predictive Value, Negative,Predictive Value, Positive
D001936 Moraxella catarrhalis Gram-negative aerobic cocci of low virulence that colonize the nasopharynx and occasionally cause MENINGITIS; BACTEREMIA; EMPYEMA; PERICARDITIS; and PNEUMONIA. Branhamella catarrhalis,Mikrokkokus catarrhalis,Moraxella (Branhamella) catarrhalis
D050260 Carbohydrate Metabolism Cellular processes in biosynthesis (anabolism) and degradation (catabolism) of CARBOHYDRATES. Metabolism, Carbohydrate

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