BIOMAT Database Logo BIOMAT Database Logo

Qualitative and Quantitative Analysis of the Saponins in Panacis Japonici Rhizoma Using Ultra-Fast Liquid Chromatography Coupled with Triple Quadrupole-Time of Flight Tandem Mass Spectrometry and Ultra-Fast Liquid Chromatography Coupled with Triple Quadrupole-Linear Ion Trap Tandem Mass Spectrometry. 2019

Jiali Chen, and Mengxia Tan, and Lisi Zou, and Xunhong Liu, and Shuyu Chen, and Jingjing Shi, and Cuihua Chen, and Chengcheng Wang, and Yuqi Mei
College of Pharmacy, Nanjing University of Chinese Medicine.

Panacis Japonici Rhizoma (PJR) contains various kinds of saponins, which possesses extensive pharmacological activities, but studies of comprehensive analysis of its saponins were limited. Thus, ultra-fast liquid chromatography coupled with triple quadrupole-time of flight tandem mass spectrometry (UFLC-Triple TOF-MS/MS) and ultra-fast liquid chromatography coupled with triple quadrupole-linear ion trap tandem mass spectrometry (UFLC-QTRAP-MS/MS) methods were established for the qualitative and quantitative analysis of the saponins in PJR, separately. Fifty three saponins in PJR were identified by UFLC-Triple TOF-MS/MS method, 23 saponins of which were unequivocally identified by reference substances. In addition, fragmentation pathways of different types of saponins were preliminarily deduced by fragmentation behavior of 53 saponins. Furthermore, the simultaneous determination of the contents of 13 saponins in PJR samples harvested at different times were analyzed by UFLC-QTRAP-MS/MS method. Furthermore, the quality of the samples was evaluated by grey relational analysis. This study might be beneficial to the quality assessment and control of PJR. Meanwhile, it might provide the basic information for confirming its optimal harvested period.

UI MeSH Term Description Entries
D008968 Molecular Conformation The characteristic three-dimensional shape of a molecule. Molecular Configuration,3D Molecular Structure,Configuration, Molecular,Molecular Structure, Three Dimensional,Three Dimensional Molecular Structure,3D Molecular Structures,Configurations, Molecular,Conformation, Molecular,Conformations, Molecular,Molecular Configurations,Molecular Conformations,Molecular Structure, 3D,Molecular Structures, 3D,Structure, 3D Molecular,Structures, 3D Molecular
D002853 Chromatography, Liquid Chromatographic techniques in which the mobile phase is a liquid. Liquid Chromatography
D012503 Saponins A type of glycoside widely distributed in plants. Each consists of a sapogenin as the aglycone moiety, and a sugar. The sapogenin may be a steroid or a triterpene and the sugar may be glucose, galactose, a pentose, or a methylpentose. Saponin
D013997 Time Factors Elements of limited time intervals, contributing to particular results or situations. Time Series,Factor, Time,Time Factor
D053719 Tandem Mass Spectrometry A mass spectrometry technique using two (MS/MS) or more mass analyzers. With two in tandem, the precursor ions are mass-selected by a first mass analyzer, and focused into a collision region where they are then fragmented into product ions which are then characterized by a second mass analyzer. A variety of techniques are used to separate the compounds, ionize them, and introduce them to the first mass analyzer. For example, for in GC-MS/MS, GAS CHROMATOGRAPHY-MASS SPECTROMETRY is involved in separating relatively small compounds by GAS CHROMATOGRAPHY prior to injecting them into an ionization chamber for the mass selection. Mass Spectrometry-Mass Spectrometry,Mass Spectrometry Mass Spectrometry,Mass Spectrometry, Tandem
D027343 Rhizome Root-like underground horizontal stem of plants that produces shoots above and roots below. Distinguished from true roots which don't have buds and nodes. Similar to true roots in being underground and thickened by storage deposits. Rhizomes

Related Publications

Jiali Chen, and Mengxia Tan, and Lisi Zou, and Xunhong Liu, and Shuyu Chen, and Jingjing Shi, and Cuihua Chen, and Chengcheng Wang, and Yuqi Mei
Comprehensive Characterization of Triterpene Saponins in Rhizoma Panacis Japonici by Offline Two-Dimensional Liquid Chromatography Coupled to Quadrupole Time-of-Flight Mass Spectrometry.
March 2024, Molecules (Basel, Switzerland),
Jiali Chen, and Mengxia Tan, and Lisi Zou, and Xunhong Liu, and Shuyu Chen, and Jingjing Shi, and Cuihua Chen, and Chengcheng Wang, and Yuqi Mei
Systematic Qualitative and Quantitative Analyses of Wenxin Granule via Ultra-High Performance Liquid Chromatography Coupled with Ion Mobility Quadrupole Time-of-Flight Mass Spectrometry and Triple Quadrupole-Linear Ion Trap Mass Spectrometry.
June 2022, Molecules (Basel, Switzerland),
Jiali Chen, and Mengxia Tan, and Lisi Zou, and Xunhong Liu, and Shuyu Chen, and Jingjing Shi, and Cuihua Chen, and Chengcheng Wang, and Yuqi Mei
Quantitative analysis of 17 hypoglycemic drugs in fingerprints using ultra-high-performance liquid chromatography/tandem hybrid triple quadrupole linear ion trap mass spectrometry.
January 2022, Rapid communications in mass spectrometry : RCM,
Jiali Chen, and Mengxia Tan, and Lisi Zou, and Xunhong Liu, and Shuyu Chen, and Jingjing Shi, and Cuihua Chen, and Chengcheng Wang, and Yuqi Mei
Study of the in vitro metabolism of TJ0711 using ultra high performance liquid chromatography with quadrupole time-of-flight and ultra fast liquid chromatography with quadrupole linear ion trap mass spectrometry.
June 2015, Journal of separation science,
Jiali Chen, and Mengxia Tan, and Lisi Zou, and Xunhong Liu, and Shuyu Chen, and Jingjing Shi, and Cuihua Chen, and Chengcheng Wang, and Yuqi Mei
Identification of metabolites of anticancer candidate salinomycin using liquid chromatography coupled with quadrupole time-of-flight and hybrid triple quadrupole linear ion trap mass spectrometry.
April 2018, Rapid communications in mass spectrometry : RCM,
Jiali Chen, and Mengxia Tan, and Lisi Zou, and Xunhong Liu, and Shuyu Chen, and Jingjing Shi, and Cuihua Chen, and Chengcheng Wang, and Yuqi Mei
[Qualitative and quantitative analysis of amygdalin and its metabolite prunasin in plasma by ultra-high performance liquid chromatography-tandem quadrupole time of flight mass spectrometry and ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry].
June 2014, Se pu = Chinese journal of chromatography,
Jiali Chen, and Mengxia Tan, and Lisi Zou, and Xunhong Liu, and Shuyu Chen, and Jingjing Shi, and Cuihua Chen, and Chengcheng Wang, and Yuqi Mei
Qualitative and Quantitative Analysis of Major Triterpenoids in Alismatis Rhizoma by High Performance Liquid Chromatography/Diode-Array Detector/Quadrupole-Time-of-Flight Mass Spectrometry and Ultra-Performance Liquid Chromatography/Triple Quadrupole Mass Spectrometry.
July 2015, Molecules (Basel, Switzerland),
Jiali Chen, and Mengxia Tan, and Lisi Zou, and Xunhong Liu, and Shuyu Chen, and Jingjing Shi, and Cuihua Chen, and Chengcheng Wang, and Yuqi Mei
Simultaneous Qualitative and Quantitative Analysis of Multiple Chemical Constituents in YiQiFuMai Injection by Ultra-Fast Liquid Chromatography Coupled with Ion Trap Time-of-Flight Mass Spectrometry.
May 2016, Molecules (Basel, Switzerland),
Jiali Chen, and Mengxia Tan, and Lisi Zou, and Xunhong Liu, and Shuyu Chen, and Jingjing Shi, and Cuihua Chen, and Chengcheng Wang, and Yuqi Mei
Metabolite Profile of Salidroside in Rats by Ultraperformance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry and High-Performance Liquid Chromatography Coupled with Quadrupole-Linear Ion Trap Mass Spectrometry.
October 2015, Journal of agricultural and food chemistry,
Jiali Chen, and Mengxia Tan, and Lisi Zou, and Xunhong Liu, and Shuyu Chen, and Jingjing Shi, and Cuihua Chen, and Chengcheng Wang, and Yuqi Mei
Analysis of anthelmintics in surface water by ultra high performance liquid chromatography coupled to quadrupole linear ion trap tandem mass spectrometry.
March 2014, Chemosphere,
Copied contents to your clipboard!