BIOMAT Database Logo BIOMAT Database Logo

Monoclonal antibodies which identify a genus-specific Listeria antigen. 1988

B T Butman, and M C Plank, and R J Durham, and J A Mattingly
Bionetics Research Inst., Rockville, Maryland 20850-4373.

Fifteen murine monoclonal antibodies (MAbs) which react specifically with a protein antigen found in all species of Listeria were developed and characterized. These MAbs were tested extensively by both enzyme-linked immunosorbent assay and Western blot (immunoblot) analyses for cross-reaction with non-Listeria organisms, such as Staphylococcus, Streptococcus, Citrobacter, Pseudomonas, and Salmonella species, and were found to be nonreactive. The genus-specific antigen was identified as a heat-stable protein with a molecular weight in the range of 30,000 to 38,000 (under both reducing and nonreducing conditions), depending on the species of Listeria tested. In Listeria monocytogenes, L. innocua, L. ivanovii, and L. seeligeri the antigen has a molecular weight of approximately 30,000 to 34,000. In L. grayi and L. murrayi it has a molecular weight of approximately 35,000 to 38,000. In addition, several of the MAbs recognize lower-molecular-weight protein bands. There appear to be at least two groups of Listeria-specific MAbs based upon isotype and results of enzyme-linked immunosorbent assay and Western blot analyses. These MAbs have proven to be useful in the development of a diagnostic assay for Listeria species in food products.

UI MeSH Term Description Entries
D007118 Immunoassay A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance. Immunochromatographic Assay,Assay, Immunochromatographic,Assays, Immunochromatographic,Immunoassays,Immunochromatographic Assays
D008087 Listeria A genus of bacteria which may be found in the feces of animals and man, on vegetation, and in silage. Its species are parasitic on cold-blooded and warm-blooded animals, including man.
D008089 Listeria monocytogenes A species of gram-positive, rod-shaped bacteria widely distributed in nature. It has been isolated from sewage, soil, silage, and from feces of healthy animals and man. Infection with this bacterium leads to encephalitis, meningitis, endocarditis, and abortion.
D008970 Molecular Weight The sum of the weight of all the atoms in a molecule. Molecular Weights,Weight, Molecular,Weights, Molecular
D003429 Cross Reactions Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen. Cross Reaction,Reaction, Cross,Reactions, Cross
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D006825 Hybridomas Cells artificially created by fusion of activated lymphocytes with neoplastic cells. The resulting hybrid cells are cloned and produce pure MONOCLONAL ANTIBODIES or T-cell products, identical to those produced by the immunologically competent parent cell. Hybridoma
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000907 Antibodies, Bacterial Immunoglobulins produced in a response to BACTERIAL ANTIGENS. Bacterial Antibodies
D000911 Antibodies, Monoclonal Antibodies produced by a single clone of cells. Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal

Related Publications

B T Butman, and M C Plank, and R J Durham, and J A Mattingly
Development and characterization of monoclonal antibodies specific for the genus Listeria.
June 1995, FEMS immunology and medical microbiology,
B T Butman, and M C Plank, and R J Durham, and J A Mattingly
Detection of alphaviruses in a genus-specific antigen capture enzyme immunoassay using monoclonal antibodies.
January 1991, Journal of clinical microbiology,
B T Butman, and M C Plank, and R J Durham, and J A Mattingly
Production of Bartonella genus-specific monoclonal antibodies.
July 2001, Clinical and diagnostic laboratory immunology,
B T Butman, and M C Plank, and R J Durham, and J A Mattingly
Human monoclonal antibodies to a genus-specific chlamydial antigen, produced by EBV-transformed B cells.
June 1983, Journal of immunology (Baltimore, Md. : 1950),
B T Butman, and M C Plank, and R J Durham, and J A Mattingly
Monoclonal antibodies which identify carbohydrate-defined MHC class I epitopes.
June 1991, Immunology and cell biology,
B T Butman, and M C Plank, and R J Durham, and J A Mattingly
Immunohistochemical characterization of two monoclonal antibodies, P25.48 and P25.91, which define a new prostate-specific antigen.
December 1988, Cancer research,
B T Butman, and M C Plank, and R J Durham, and J A Mattingly
Monoclonal antibodies and prostate-specific membrane antigen.
June 2004, Current opinion in investigational drugs (London, England : 2000),
B T Butman, and M C Plank, and R J Durham, and J A Mattingly
Production of monoclonal antibodies against leptospiral genus-specific protein antigen and localization of the antigen by immunoelectron microscopy.
July 1985, Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology,
B T Butman, and M C Plank, and R J Durham, and J A Mattingly
Comparison of Two Strategies to Generate Antigen-Specific Human Monoclonal Antibodies: Which Method to Choose for Which Purpose?
January 2021, Frontiers in immunology,
B T Butman, and M C Plank, and R J Durham, and J A Mattingly
Monoclonal antibodies specific for members of the genus Endotrypanum.
January 1989, The Journal of protozoology,
Copied contents to your clipboard!