Methylcellulose inhibition of exo-beta-1,4-glucanase A from Ruminococcus flavefaciens FD-1. 1988

B A White, and M A Rasmussen, and R M Gardner
Department of Animal Sciences, University of Illinois, Urbana-Champaign 61801.

A homogeneous preparation of exo-beta-1,4-glucanase A from Ruminococcus flavefaciens FD-1 was competitively inhibited by low concentrations (less than 3 mM) of methylcellulose. The enzyme was also sensitive to the surfactant properties of methylcellulose at high methylcellulose concentrations.

UI MeSH Term Description Entries
D007700 Kinetics The rate dynamics in chemical or physical systems.
D008747 Methylcellulose Methylester of cellulose. Methylcellulose is used as an emulsifying and suspending agent in cosmetics, pharmaceutics and the chemical industry. It is used therapeutically as a bulk laxative. BFL,Celevac,Cellothyl,Cellulone,Citrucel,Cologel,Dacryolarmes,Methocel,Methyl Cellulose,Muciplasma,Cellulose, Methyl
D010459 Peptococcaceae A family of bacteria found in the mouth and intestinal and respiratory tracts of man and other animals as well as in the human female urogenital tract. Its organisms are also found in soil and on cereal grains.
D005959 Glucosidases Enzymes that hydrolyze O-glucosyl-compounds. (Enzyme Nomenclature, 1992) EC 3.2.1.-. Glucosidase
D006868 Hydrolysis The process of cleaving a chemical compound by the addition of a molecule of water.
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001617 beta-Glucosidase An exocellulase with specificity for a variety of beta-D-glycoside substrates. It catalyzes the hydrolysis of terminal non-reducing residues in beta-D-glucosides with release of GLUCOSE. Cellobiases,Amygdalase,Cellobiase,Emulsion beta-D-Glucosidase,Gentiobiase,Emulsion beta D Glucosidase,beta Glucosidase,beta-D-Glucosidase, Emulsion
D013501 Surface-Active Agents Agents that modify interfacial tension of water; usually substances that have one lipophilic and one hydrophilic group in the molecule; includes soaps, detergents, emulsifiers, dispersing and wetting agents, and several groups of antiseptics. Surface Active Agent,Surface-Active Agent,Surfactant,Surfactants,Tenside,Amphiphilic Agents,Surface Active Agents,Tensides,Active Agent, Surface,Active Agents, Surface,Agent, Surface Active,Agent, Surface-Active,Agents, Amphiphilic,Agents, Surface Active,Agents, Surface-Active
D044643 Glucan 1,4-beta-Glucosidase An exocellulase with specificity for the hydrolysis of 1,4-beta-glucosidic linkages of 1,4-beta-D-glucans resulting in successive removal of GLUCOSE units. 1,4-beta-D-Glucan Glucohydrolase,Exo-1,4-beta-Glucosidase,Exo-beta-1,4-Glucanase,beta-(1-4)-Glucanase,1,4 beta D Glucan Glucohydrolase,1,4-beta-Glucosidase, Glucan,Exo 1,4 beta Glucosidase,Exo beta 1,4 Glucanase,Glucan 1,4 beta Glucosidase,Glucohydrolase, 1,4-beta-D-Glucan

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