Biomaterial Database

Glycated lysine increases in serum samples stored at -20 degrees C. 1988

L Schäffer

Department of Clinical Biochemistry, Novo Research Institute, Bagsvaerd, Denmark.

The glycated lysine in serum protein was determined by HPLC measurement of furosine in hydrolyzed serum, with synthetic N alpha-Boc-N epsilon-(1-deoxy-D-fructosyl)-L-lysine as the standard. The glycated lysine value increased with time of storage when the serum was kept at -20 degrees C, possibly because of an ongoing reaction between glucose and the epsilon-amino groups of lysine in the frozen serum. The problem was eliminated by using storage at -70 degrees C or by acidifying the serum before storing it at -20 degrees C.

UI	MeSH Term	Description	Entries
D008239	Lysine	An essential amino acid. It is often added to animal feed.	Enisyl,L-Lysine,Lysine Acetate,Lysine Hydrochloride,Acetate, Lysine,L Lysine
D001798	Blood Proteins	Proteins that are present in blood serum, including SERUM ALBUMIN; BLOOD COAGULATION FACTORS; and many other types of proteins.	Blood Protein,Plasma Protein,Plasma Proteins,Serum Protein,Serum Proteins,Protein, Blood,Protein, Plasma,Protein, Serum,Proteins, Blood,Proteins, Plasma,Proteins, Serum
D002851	Chromatography, High Pressure Liquid	Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.	Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D002951	Citrates	Derivatives of CITRIC ACID.
D003920	Diabetes Mellitus	A heterogeneous group of disorders characterized by HYPERGLYCEMIA and GLUCOSE INTOLERANCE.
D005615	Freezing	Liquids transforming into solids by the removal of heat.	Melting
D006023	Glycoproteins	Conjugated protein-carbohydrate compounds including MUCINS; mucoid, and AMYLOID glycoproteins.	C-Glycosylated Proteins,Glycosylated Protein,Glycosylated Proteins,N-Glycosylated Proteins,O-Glycosylated Proteins,Glycoprotein,Neoglycoproteins,Protein, Glycosylated,Proteins, C-Glycosylated,Proteins, Glycosylated,Proteins, N-Glycosylated,Proteins, O-Glycosylated
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006863	Hydrogen-Ion Concentration	The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH	pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D000093422	Glycated Serum Proteins	Proteins in serum that have undergone, in vivo, non-enzymatic modification with reducing sugars via the MAILLARD REACTION. They are analyzed to measure control of HYPERGLYCEMIA.	Glycated Blood Protein,Glycated Blood Proteins,Glycated Serum Protein,Glycosylated Blood Protein,Glycosylated Blood Proteins,Glycosylated Serum Protein,Glycosylated Serum Proteins,Serum Protein, Glycated,Serum Protein, Glycosylated,Blood Protein, Glycated,Blood Protein, Glycosylated,Blood Proteins, Glycated,Blood Proteins, Glycosylated,Protein, Glycated Blood,Protein, Glycosylated Blood,Proteins, Glycated Blood,Proteins, Glycosylated Blood,Proteins, Glycosylated Serum,Serum Proteins, Glycated,Serum Proteins, Glycosylated