BIOMAT Database Logo BIOMAT Database Logo

Dihydroartemisinin inhibits HepG2.2.15 proliferation by inducing cellular senescence and autophagy. 2019

Jiang Zou, and Qiang Ma, and Ru Sun, and Jiajing Cai, and Hebin Liao, and Lei Xu, and Jingruo Xia, and Guangcheng Huang, and Lihua Yao, and Yan Cai, and Xiaowu Zhong, and Xiaolan Guo
Department of Clinical Laboratory, Affiliated Hospital of North Sichuan Medical College; Translational Medicine Research Center, North Sichuan Medical College; Department of Laboratory Medicine, North Sichuan Medical College, Nanchong 637000, P.R.China.

Dihydroartemisinin (DHA) has been reported to possess anti-cancer activity against many cancers. However, the pharmacologic effect of DHA on HBV-positive hepatocellular carcinoma (HCC) remains unknown. Thus, the objective of the present study was to determine whether DHA could inhibit the proliferation of HepG2.2.15 cells and uncover the underlying mechanisms involved in the effect of DHA on HepG2.2.15 cells. We found that DHA effectively inhibited HepG2.2.15 HCC cell proliferation both in vivo and in vitro. DHA also reduced the migration and tumorigenicity capacity of HepG2.2.15 cells. Regarding the underlying mechanisms, results showed that DHA induced cellular senescence by up-regulating expression levels of proteins such as p-ATM, p-ATR, γ-H2AX, P53, and P21 involved in DNA damage response. DHA also induced autophagy (green LC3 puncta gathered together and LC3II/LC3I ratio increased through AKT-mTOR pathway suppression). Results also revealed that DHA-induced autophagy was not linked to senescence or cell death. TPP1 (telomere shelterin) overexpression could not rescue DHA-induced anticancer activity (cell proliferation). Moreover, DHA down-regulated TPP1 expression. Gene knockdown of TPP1 caused similar phenotypes and mechanisms as DHA induced phenotypes and mechanisms in HepG2.2.15 cells. These results demonstrate that DHA might inhibit HepG2.2.15 cells proliferation through inducing cellular senescence and autophagy. [BMB Reports 2019; 52(8): 520-524].

UI MeSH Term Description Entries
D002465 Cell Movement The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell. Cell Migration,Locomotion, Cell,Migration, Cell,Motility, Cell,Movement, Cell,Cell Locomotion,Cell Motility,Cell Movements,Movements, Cell
D004305 Dose-Response Relationship, Drug The relationship between the dose of an administered drug and the response of the organism to the drug. Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D004354 Drug Screening Assays, Antitumor Methods of investigating the effectiveness of anticancer cytotoxic drugs and biologic inhibitors. These include in vitro cell-kill models and cytostatic dye exclusion tests as well as in vivo measurement of tumor growth parameters in laboratory animals. Anticancer Drug Sensitivity Tests,Antitumor Drug Screens,Cancer Drug Tests,Drug Screening Tests, Tumor-Specific,Dye Exclusion Assays, Antitumor,Anti-Cancer Drug Screens,Antitumor Drug Screening Assays,Tumor-Specific Drug Screening Tests,Anti Cancer Drug Screens,Anti-Cancer Drug Screen,Antitumor Drug Screen,Cancer Drug Test,Drug Screen, Anti-Cancer,Drug Screen, Antitumor,Drug Screening Tests, Tumor Specific,Drug Screens, Anti-Cancer,Drug Screens, Antitumor,Drug Test, Cancer,Drug Tests, Cancer,Screen, Anti-Cancer Drug,Screen, Antitumor Drug,Screens, Anti-Cancer Drug,Screens, Antitumor Drug,Test, Cancer Drug,Tests, Cancer Drug,Tumor Specific Drug Screening Tests
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000089804 Shelterin Complex A TELOMERE cap complex consisting of telomere-specific proteins in association with telomeric DNA such as telomeric dsDNA-sDNA junction. They are involved in the protection of chromosome ends and TELOMERASE regulation and play a role in CELLULAR SENESCENCE and ageing-related pathology. In general it consists of six mostly TELOMERE-BINDING PROTEINS (POT1, RAP1, TIN2, TPP1, TRF1, and TRF2). CST Complex,Ctc1-Stn1-Ten1 Complex,POT1-TPP1 Shelterin Complex,Telomere Cap Complex,Telomere POT1-TPP1 Complex,Telomeric Capping Complex,Telomeric Stn1-Ten1 Capping Complex,Telosome,Capping Complex, Telomeric,Complex, CST,Complex, Ctc1-Stn1-Ten1,Complex, POT1-TPP1 Shelterin,Complex, Shelterin,Complex, Telomere POT1-TPP1,Complex, Telomeric Capping,Ctc1 Stn1 Ten1 Complex,POT1 TPP1 Shelterin Complex,POT1-TPP1 Complex, Telomere,Shelterin Complex, POT1-TPP1,Telomere POT1 TPP1 Complex,Telomeric Stn1 Ten1 Capping Complex,Telosomes
D000970 Antineoplastic Agents Substances that inhibit or prevent the proliferation of NEOPLASMS. Anticancer Agent,Antineoplastic,Antineoplastic Agent,Antineoplastic Drug,Antitumor Agent,Antitumor Drug,Cancer Chemotherapy Agent,Cancer Chemotherapy Drug,Anticancer Agents,Antineoplastic Drugs,Antineoplastics,Antitumor Agents,Antitumor Drugs,Cancer Chemotherapy Agents,Cancer Chemotherapy Drugs,Chemotherapeutic Anticancer Agents,Chemotherapeutic Anticancer Drug,Agent, Anticancer,Agent, Antineoplastic,Agent, Antitumor,Agent, Cancer Chemotherapy,Agents, Anticancer,Agents, Antineoplastic,Agents, Antitumor,Agents, Cancer Chemotherapy,Agents, Chemotherapeutic Anticancer,Chemotherapy Agent, Cancer,Chemotherapy Agents, Cancer,Chemotherapy Drug, Cancer,Chemotherapy Drugs, Cancer,Drug, Antineoplastic,Drug, Antitumor,Drug, Cancer Chemotherapy,Drug, Chemotherapeutic Anticancer,Drugs, Antineoplastic,Drugs, Antitumor,Drugs, Cancer Chemotherapy
D001343 Autophagy The segregation and degradation of various cytoplasmic constituents via engulfment by MULTIVESICULAR BODIES; VACUOLES; or AUTOPHAGOSOMES and their digestion by LYSOSOMES. It plays an important role in BIOLOGICAL METAMORPHOSIS and in the removal of bone by OSTEOCLASTS. Defective autophagy is associated with various diseases, including NEURODEGENERATIVE DISEASES and cancer. Autophagocytosis,ER-Phagy,Lipophagy,Nucleophagy,Reticulophagy,Ribophagy,Autophagy, Cellular,Cellular Autophagy,ER Phagy
D013329 Structure-Activity Relationship The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups. Relationship, Structure-Activity,Relationships, Structure-Activity,Structure Activity Relationship,Structure-Activity Relationships
D016922 Cellular Senescence Process by which cells irreversibly stop dividing and enter a state of permanent growth arrest without undergoing CELL DEATH. Senescence can be induced by DNA DAMAGE or other cellular stresses, such as OXIDATIVE STRESS. Aging, Cell,Cell Aging,Cell Senescence,Replicative Senescence,Senescence, Cellular,Senescence, Replicative,Cell Ageing,Cellular Ageing,Cellular Aging,Ageing, Cell,Ageing, Cellular,Aging, Cellular,Senescence, Cell
D049109 Cell Proliferation All of the processes involved in increasing CELL NUMBER including CELL DIVISION. Cell Growth in Number,Cellular Proliferation,Cell Multiplication,Cell Number Growth,Growth, Cell Number,Multiplication, Cell,Number Growth, Cell,Proliferation, Cell,Proliferation, Cellular

Related Publications

Jiang Zou, and Qiang Ma, and Ru Sun, and Jiajing Cai, and Hebin Liao, and Lei Xu, and Jingruo Xia, and Guangcheng Huang, and Lihua Yao, and Yan Cai, and Xiaowu Zhong, and Xiaolan Guo
Dihydroartemisinin inhibits the migration of esophageal cancer cells by inducing autophagy.
October 2020, Oncology letters,
Jiang Zou, and Qiang Ma, and Ru Sun, and Jiajing Cai, and Hebin Liao, and Lei Xu, and Jingruo Xia, and Guangcheng Huang, and Lihua Yao, and Yan Cai, and Xiaowu Zhong, and Xiaolan Guo
EVA1A inhibits GBM cell proliferation by inducing autophagy and apoptosis.
March 2017, Experimental cell research,
Jiang Zou, and Qiang Ma, and Ru Sun, and Jiajing Cai, and Hebin Liao, and Lei Xu, and Jingruo Xia, and Guangcheng Huang, and Lihua Yao, and Yan Cai, and Xiaowu Zhong, and Xiaolan Guo
How autophagy both activates and inhibits cellular senescence.
May 2016, Autophagy,
Jiang Zou, and Qiang Ma, and Ru Sun, and Jiajing Cai, and Hebin Liao, and Lei Xu, and Jingruo Xia, and Guangcheng Huang, and Lihua Yao, and Yan Cai, and Xiaowu Zhong, and Xiaolan Guo
Ginsenoside Rb2 suppresses cellular senescence of human dermal fibroblasts by inducing autophagy.
March 2023, Journal of ginseng research,
Jiang Zou, and Qiang Ma, and Ru Sun, and Jiajing Cai, and Hebin Liao, and Lei Xu, and Jingruo Xia, and Guangcheng Huang, and Lihua Yao, and Yan Cai, and Xiaowu Zhong, and Xiaolan Guo
Dihydroartemisinin Inhibits TGF-β-Induced Fibrosis in Human Tenon Fibroblasts via Inducing Autophagy.
January 2021, Drug design, development and therapy,
Jiang Zou, and Qiang Ma, and Ru Sun, and Jiajing Cai, and Hebin Liao, and Lei Xu, and Jingruo Xia, and Guangcheng Huang, and Lihua Yao, and Yan Cai, and Xiaowu Zhong, and Xiaolan Guo
Gypenoside L Inhibits Proliferation of Liver and Esophageal Cancer Cells by Inducing Senescence.
March 2019, Molecules (Basel, Switzerland),
Jiang Zou, and Qiang Ma, and Ru Sun, and Jiajing Cai, and Hebin Liao, and Lei Xu, and Jingruo Xia, and Guangcheng Huang, and Lihua Yao, and Yan Cai, and Xiaowu Zhong, and Xiaolan Guo
Inhibition of Clusterin Represses Proliferation by Inducing Cellular Senescence in Pancreatic Cancer.
August 2022, Annals of surgical oncology,
Jiang Zou, and Qiang Ma, and Ru Sun, and Jiajing Cai, and Hebin Liao, and Lei Xu, and Jingruo Xia, and Guangcheng Huang, and Lihua Yao, and Yan Cai, and Xiaowu Zhong, and Xiaolan Guo
Autophagy suppresses melanoma tumorigenesis by inducing senescence.
February 2014, Autophagy,
Jiang Zou, and Qiang Ma, and Ru Sun, and Jiajing Cai, and Hebin Liao, and Lei Xu, and Jingruo Xia, and Guangcheng Huang, and Lihua Yao, and Yan Cai, and Xiaowu Zhong, and Xiaolan Guo
Dihydroartemisinin increases temozolomide efficacy in glioma cells by inducing autophagy.
July 2015, Oncology letters,
Jiang Zou, and Qiang Ma, and Ru Sun, and Jiajing Cai, and Hebin Liao, and Lei Xu, and Jingruo Xia, and Guangcheng Huang, and Lihua Yao, and Yan Cai, and Xiaowu Zhong, and Xiaolan Guo
Sakurasosaponin inhibits lung cancer cell proliferation by inducing autophagy via AMPK activation.
December 2023, Oncology letters,
Copied contents to your clipboard!