BIOMAT Database Logo BIOMAT Database Logo

5'-nucleotidase of B and T lymphocytes isolated from human peripheral blood. 1979

M Rowe, and C G de Gast, and T A Platts-Mills, and G L Asherson, and A D Webster, and S M Johnson

The ecto-5'-nucleotidase activities of highly purified T and B lymphocytes from human peripheral blood have been investigated using biochemical and histochemical techniques. The enzyme activity of the purified B cells was about 3.5 times that of the T cells. Using a histochemical assay, 21--55% of the B cells stained positively for 5'-nucleotidase, but only 2--22% of the T cells were positive. These results are discussed in relation to the low 5'-nucleotidase activities found on peripheral blood lymphocytes from patients with chronic lymphatic leukaemia and some patients with primary hypogammaglobulinaemia.

UI MeSH Term Description Entries
D007945 Leukemia, Lymphoid Leukemia associated with HYPERPLASIA of the lymphoid tissues and increased numbers of circulating malignant LYMPHOCYTES and lymphoblasts. Leukemia, Lymphocytic,Lymphocytic Leukemia,Lymphoid Leukemia,Leukemias, Lymphocytic,Leukemias, Lymphoid,Lymphocytic Leukemias,Lymphoid Leukemias
D009708 Nucleotidases A class of enzymes that catalyze the conversion of a nucleotide and water to a nucleoside and orthophosphate. EC 3.1.3.-.
D012016 Reference Values The range or frequency distribution of a measurement in a population (of organisms, organs or things) that has not been selected for the presence of disease or abnormality. Normal Range,Normal Values,Reference Ranges,Normal Ranges,Normal Value,Range, Normal,Range, Reference,Ranges, Normal,Ranges, Reference,Reference Range,Reference Value,Value, Normal,Value, Reference,Values, Normal,Values, Reference
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D006651 Histocytochemistry Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods. Cytochemistry
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000361 Agammaglobulinemia An immunologic deficiency state characterized by an extremely low level of generally all classes of gamma-globulin in the blood. Hypogammaglobulinemia,Agammaglobulinemias,Hypogammaglobulinemias
D001402 B-Lymphocytes Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation. B-Cells, Lymphocyte,B-Lymphocyte,Bursa-Dependent Lymphocytes,B Cells, Lymphocyte,B Lymphocyte,B Lymphocytes,B-Cell, Lymphocyte,Bursa Dependent Lymphocytes,Bursa-Dependent Lymphocyte,Lymphocyte B-Cell,Lymphocyte B-Cells,Lymphocyte, Bursa-Dependent,Lymphocytes, Bursa-Dependent
D013601 T-Lymphocytes Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen. T Cell,T Lymphocyte,T-Cells,Thymus-Dependent Lymphocytes,Cell, T,Cells, T,Lymphocyte, T,Lymphocyte, Thymus-Dependent,Lymphocytes, T,Lymphocytes, Thymus-Dependent,T Cells,T Lymphocytes,T-Cell,T-Lymphocyte,Thymus Dependent Lymphocytes,Thymus-Dependent Lymphocyte

Related Publications

M Rowe, and C G de Gast, and T A Platts-Mills, and G L Asherson, and A D Webster, and S M Johnson
Quantitation of human peripheral blood T and B lymphocytes.
January 1975, International archives of allergy and applied immunology,
M Rowe, and C G de Gast, and T A Platts-Mills, and G L Asherson, and A D Webster, and S M Johnson
Shared idiotypes of human peripheral blood B and T lymphocytes.
June 1979, Journal of immunology (Baltimore, Md. : 1950),
M Rowe, and C G de Gast, and T A Platts-Mills, and G L Asherson, and A D Webster, and S M Johnson
[Determination of B and T lymphocytes in human peripheral blood].
June 1974, Polskie Archiwum Medycyny Wewnetrznej,
M Rowe, and C G de Gast, and T A Platts-Mills, and G L Asherson, and A D Webster, and S M Johnson
Anti-5'-nucleotidase antibodies cause human peripheral blood T cells to proliferate.
January 1989, Advances in experimental medicine and biology,
M Rowe, and C G de Gast, and T A Platts-Mills, and G L Asherson, and A D Webster, and S M Johnson
Expression of the 5'-nucleotidase gene in the peripheral blood lymphocytes from B-chronic lymphocytic leukemia. Evaluation of mRNA.
February 1996, Biochemical Society transactions,
M Rowe, and C G de Gast, and T A Platts-Mills, and G L Asherson, and A D Webster, and S M Johnson
Nucleotidase activities of human peripheral lymphocytes.
September 1985, Biochemical pharmacology,
M Rowe, and C G de Gast, and T A Platts-Mills, and G L Asherson, and A D Webster, and S M Johnson
Specificity of gamma delta receptor-bearing cytotoxic T lymphocytes isolated from human peripheral blood.
June 1989, Scandinavian journal of immunology,
M Rowe, and C G de Gast, and T A Platts-Mills, and G L Asherson, and A D Webster, and S M Johnson
Extraction and purification of ecto-5'-nucleotidase from human lymphocytes.
January 2000, Advances in experimental medicine and biology,
M Rowe, and C G de Gast, and T A Platts-Mills, and G L Asherson, and A D Webster, and S M Johnson
Functional characterization of ecto-5'-nucleotidase-positive and -negative human T lymphocytes.
March 1989, Journal of immunology (Baltimore, Md. : 1950),
M Rowe, and C G de Gast, and T A Platts-Mills, and G L Asherson, and A D Webster, and S M Johnson
Electron microscopic morphometric analysis of human T and B peripheral blood lymphocytes.
July 1978, Virchows Archiv. B, Cell pathology,
Copied contents to your clipboard!