-transparent.png){kind=logo}
Anhydrotetracycline oxygenase was purified to homogeneity from Streptomyces aureofaciens, a producer of tetracycline. The enzyme was purified 60-fold in a 40% yield by a two-step procedure using a combination of hydrophobic chromatography and ion-exchange h.p.l.c. Purified anhydrotetracycline oxygenase was homogeneous according to SDS/polyacrylamide-gel electrophoresis, isoelectric focusing, ion-exchange h.p.l.c. on a Mono Q HR 5/5 column and size-exclusion h.p.l.c. on a TSK G 3000 SW column. The enzyme consists of two subunits of Mr 57,500, as determined by SDS/polyacrylamide-gel electrophoresis.
Related Publications
I Vancurová, and
J Volc, and
M Flieger, and
J Neuzil, and
J Novotná, and
J Vlach, and
V Bĕhal
January 1978,
Folia microbiologica,
I Vancurová, and
J Volc, and
M Flieger, and
J Neuzil, and
J Novotná, and
J Vlach, and
V Bĕhal
January 1987,
Folia microbiologica,
I Vancurová, and
J Volc, and
M Flieger, and
J Neuzil, and
J Novotná, and
J Vlach, and
V Bĕhal
May 1971,
Biochimica et biophysica acta,
I Vancurová, and
J Volc, and
M Flieger, and
J Neuzil, and
J Novotná, and
J Vlach, and
V Bĕhal
March 1964,
Il Farmaco; edizione scientifica,
I Vancurová, and
J Volc, and
M Flieger, and
J Neuzil, and
J Novotná, and
J Vlach, and
V Bĕhal
January 1991,
Folia microbiologica,
I Vancurová, and
J Volc, and
M Flieger, and
J Neuzil, and
J Novotná, and
J Vlach, and
V Bĕhal
January 1987,
Methods in enzymology,
I Vancurová, and
J Volc, and
M Flieger, and
J Neuzil, and
J Novotná, and
J Vlach, and
V Bĕhal
June 1998,
General physiology and biophysics,
I Vancurová, and
J Volc, and
M Flieger, and
J Neuzil, and
J Novotná, and
J Vlach, and
V Bĕhal
October 1993,
Nucleic acids research,
I Vancurová, and
J Volc, and
M Flieger, and
J Neuzil, and
J Novotná, and
J Vlach, and
V Bĕhal
January 2005,
Biochimica et biophysica acta,
I Vancurová, and
J Volc, and
M Flieger, and
J Neuzil, and
J Novotná, and
J Vlach, and
V Bĕhal
January 1980,
Folia microbiologica,
