Overexpression of MBD3 Improves Reprogramming of Cloned Pig Embryos. 2019

Xingwang Wang, and Junsong Shi, and Gengyuan Cai, and Enqin Zheng, and Dewu Liu, and Zhenfang Wu, and Zicong Li
National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, China.

Methyl-CpG-binding domain protein 3 (MBD3) is a core component of the nucleosome remodeling and deacetylase (NuRD) complex, which is crucial for pluripotent stem cell differentiation and embryonic development. MBD3 was shown to play important roles in transcription factor-induced somatic cell reprogramming. Expression level of MBD3 was demonstrated to be higher in somatic cell nuclear transfer-generated cloned pig embryos than in fertilization-derived porcine embryos. However, the functions of MBD3 in nuclear transfer-mediated somatic cell reprogramming are unknown. In this study, MBD3 was overexpressed in cloned pig embryos, and the effects of MBD3 overexpression on gene transcription, DNA methylation, and in vitro developmental competence of cloned pig embryos were analyzed. Results indicated that overexpression of MBD3 in cloned pig embryos not only increased blastocyst rate and number of cells per blastocyst but also upregulated mRNA expression levels and decreased the DNA methylation of NANOG, OCT4, and LINE1 genes to the levels close to those in in vivo fertilization-produced pig embryos. These findings suggest that overexpression of MBD3 improves reprogramming of cloned pig embryos.

UI MeSH Term Description Entries
D011247 Pregnancy The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH. Gestation,Pregnancies
D001755 Blastocyst A post-MORULA preimplantation mammalian embryo that develops from a 32-cell stage into a fluid-filled hollow ball of over a hundred cells. A blastocyst has two distinctive tissues. The outer layer of trophoblasts gives rise to extra-embryonic tissues. The inner cell mass gives rise to the embryonic disc and eventual embryo proper. Embryo, Preimplantation,Blastocysts,Embryos, Preimplantation,Preimplantation Embryo,Preimplantation Embryos
D002454 Cell Differentiation Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs. Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D004268 DNA-Binding Proteins Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases. DNA Helix Destabilizing Proteins,DNA-Binding Protein,Single-Stranded DNA Binding Proteins,DNA Binding Protein,DNA Single-Stranded Binding Protein,SS DNA BP,Single-Stranded DNA-Binding Protein,Binding Protein, DNA,DNA Binding Proteins,DNA Single Stranded Binding Protein,DNA-Binding Protein, Single-Stranded,Protein, DNA-Binding,Single Stranded DNA Binding Protein,Single Stranded DNA Binding Proteins
D004622 Embryo, Mammalian The entity of a developing mammal (MAMMALS), generally from the cleavage of a ZYGOTE to the end of embryonic differentiation of basic structures. For the human embryo, this represents the first two months of intrauterine development preceding the stages of the FETUS. Embryonic Structures, Mammalian,Mammalian Embryo,Mammalian Embryo Structures,Mammalian Embryonic Structures,Embryo Structure, Mammalian,Embryo Structures, Mammalian,Embryonic Structure, Mammalian,Embryos, Mammalian,Mammalian Embryo Structure,Mammalian Embryonic Structure,Mammalian Embryos,Structure, Mammalian Embryo,Structure, Mammalian Embryonic,Structures, Mammalian Embryo,Structures, Mammalian Embryonic
D005260 Female Females
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013552 Swine Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA). Phacochoerus,Pigs,Suidae,Warthogs,Wart Hogs,Hog, Wart,Hogs, Wart,Wart Hog
D044127 Epigenesis, Genetic A genetic process by which the adult organism is realized via mechanisms that lead to the restriction in the possible fates of cells, eventually leading to their differentiated state. Mechanisms involved cause heritable changes to cells without changes to DNA sequence such as DNA METHYLATION; HISTONE modification; DNA REPLICATION TIMING; NUCLEOSOME positioning; and heterochromatization which result in selective gene expression or repression. Epigenetic Processes,Epigenetic Process,Epigenetics Processes,Genetic Epigenesis,Process, Epigenetic,Processes, Epigenetic,Processes, Epigenetics
D046149 Embryo Culture Techniques The technique of maintaining or growing mammalian EMBRYOS in vitro. This method offers an opportunity to observe EMBRYONIC DEVELOPMENT; METABOLISM; and susceptibility to TERATOGENS. Blastocyst Culture Techniques,Blastocyst Culture Technique,Culture Technique, Blastocyst,Culture Technique, Embryo,Culture Techniques, Blastocyst,Culture Techniques, Embryo,Embryo Culture Technique

Related Publications

Xingwang Wang, and Junsong Shi, and Gengyuan Cai, and Enqin Zheng, and Dewu Liu, and Zhenfang Wu, and Zicong Li
June 2015, Cellular reprogramming,
Xingwang Wang, and Junsong Shi, and Gengyuan Cai, and Enqin Zheng, and Dewu Liu, and Zhenfang Wu, and Zicong Li
February 2018, Zygote (Cambridge, England),
Xingwang Wang, and Junsong Shi, and Gengyuan Cai, and Enqin Zheng, and Dewu Liu, and Zhenfang Wu, and Zicong Li
June 2018, Cellular reprogramming,
Xingwang Wang, and Junsong Shi, and Gengyuan Cai, and Enqin Zheng, and Dewu Liu, and Zhenfang Wu, and Zicong Li
January 2004, IEEE engineering in medicine and biology magazine : the quarterly magazine of the Engineering in Medicine & Biology Society,
Xingwang Wang, and Junsong Shi, and Gengyuan Cai, and Enqin Zheng, and Dewu Liu, and Zhenfang Wu, and Zicong Li
March 2017, Journal of animal science,
Xingwang Wang, and Junsong Shi, and Gengyuan Cai, and Enqin Zheng, and Dewu Liu, and Zhenfang Wu, and Zicong Li
June 2018, The Journal of reproduction and development,
Xingwang Wang, and Junsong Shi, and Gengyuan Cai, and Enqin Zheng, and Dewu Liu, and Zhenfang Wu, and Zicong Li
January 2003, Theriogenology,
Xingwang Wang, and Junsong Shi, and Gengyuan Cai, and Enqin Zheng, and Dewu Liu, and Zhenfang Wu, and Zicong Li
October 2001, The Journal of biological chemistry,
Xingwang Wang, and Junsong Shi, and Gengyuan Cai, and Enqin Zheng, and Dewu Liu, and Zhenfang Wu, and Zicong Li
November 2001, Proceedings of the National Academy of Sciences of the United States of America,
Xingwang Wang, and Junsong Shi, and Gengyuan Cai, and Enqin Zheng, and Dewu Liu, and Zhenfang Wu, and Zicong Li
March 2013, Molecular and cellular biology,
Copied contents to your clipboard!